PXD074986

PXD074986 is an original dataset announced via ProteomeXchange.

Title	In Vivo Labeling–Based Proteomic Analysis of Early Follicle Oocytes and Cisplatin-Induced Alterations in Mice
Description	This project aims to systematically characterize the proteome of oocytes predominantly from primordial follicles using an in vivo APEX-based proximity labeling strategy. Due to the rarity of primordial follicle oocytes and technical limitations in their isolation, comprehensive proteomic profiling of early-stage oocytes has remained largely unexplored. In this study, we generated transgenic APEX fluorescent mice to enable oocyte-specific protein labeling under physiological conditions. Using this system, we identified 2,772 proteins and performed quantitative analyses of proteomic alterations following short-term cisplatin exposure. The data revealed dynamic changes in pathways related to DNA damage response and histone modification. This dataset provides a valuable resource for understanding early-stage oocyte biology and chemotherapy-induced reproductive toxicity.
HostingRepository	iProX
AnnounceDate	2026-02-27
AnnouncementXML	Submission_2026-02-27_01:19:59.478.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bingteng Xie
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-27 01:19:43	ID requested
⏵ 1	2026-02-27 01:20:00	announced

Dataset with its publication pending

submitter keyword: Primordial follicles, oocytes, Proteome in vivo labeling, APEX2, Cisplatin

Bingteng Xie
contact affiliation	Beijing Institute of Technology
contact email	xiebingteng@bit.edu.cn
lab head
Bingteng Xie
contact affiliation	Beijing Institute of Technology
contact email	xiebingteng@bit.edu.cn
dataset submitter

iProX dataset URI