PXD074981

PXD074981 is an original dataset announced via ProteomeXchange.

Title	Aptamer-Mediated Proximity Labeling (ApMPL) for Spatially Resolved Deciphering Cell Membrane Protein Interactome
Description	Cell membrane protein-protein interactions (PPIs) play pivotal roles in maintaining cellular homeostasis and physiological balance. Deciphering these interactions is crucial for understanding disease mechanisms and identifying therapeutic targets. While proximity labeling technologies have revolutionized PPI studies, existing methods face limitations including operational complexity, safety concerns, uncontrollable labeling radius, and poor membrane protein accessibility. To overcome these challenges, we developed an Aptamer-Mediated Proximity Labeling (ApMPL) system that enables radius-tunable labeling and capture of membrane protein interactomes. The ApMPL probe combines aptamer-based precision targeting, adjustable interaction radii through programmable DNA spacers and efficient bioorthogonal capture of proximal proteins. Using tumor-associated membrane proteins Nucleolin as model targets, we demonstrated this innovative approach leverages the unparalleled programmability of DNA nanotechnology to systematically map membrane PPIs with accurate spatial control. Meanwhile, different interacting proteins of Nucleolin between normal physiological state and apoptotic state could also been captured. Additionally, the versatility and adaptability of the platform has been confirmed on the PTK7 protein and the FGFR2 protein. The ApMPL platform will be valuable for discovering novel membrane protein interactions, elucidating critical signaling networks and facilitating therapeutic target identification
HostingRepository	iProX
AnnounceDate	2026-02-27
AnnouncementXML	Submission_2026-02-27_01:00:01.205.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qiuxia Yang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-27 00:56:57	ID requested
⏵ 1	2026-02-27 01:00:01	announced

Dataset with its publication pending

submitter keyword: Aptamer, Spatially Resolved Proximal Labeling, Metabolic Glycoengineering, Cell Membrane Protein Interactome

Qiuxia Yang
contact affiliation	State Key Laboratory of Metabolic Dysregulation & Prevention and Treatment of Esophageal Cancer, Tianjian Laboratory of Advanced Biomedical Sciences, Academy of Medical Sciences, Zhengzhou University
contact email	yangqx2023@zzu.edu.cn
lab head
Qiuxia Yang
contact affiliation	State Key Laboratory of Metabolic Dysregulation & Prevention and Treatment of Esophageal Cancer, Tianjian Laboratory of Advanced Biomedical Sciences, Academy of Medical Sciences, Zhengzhou University
contact email	yangqx2023@zzu.edu.cn
dataset submitter

iProX dataset URI