PXD074970

PXD074970 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomic screen of PACS1 interactors in HCT116 cells
Description	PACS1 syndrome is a neurodevelopmental disorder caused by a recurrent heterozygous missense mutation in PACS1 (p.R203W). We previously showed that PACS1R203W aberrantly potentiates HDAC6 activity, leading to Golgi fragmentation and neuronal deficits through an unresolved mechanism. To elucidate how PACS1R203W and HDAC6 disrupt Golgi positioning, we performed a proteomic screen to identify additional PACS1R203W partners involved in microtubule-based trafficking. HCT116 cells expressing FLAG-tagged PACS1R203W or PACS1 (control) underwent anti-FLAG immunoprecipitation (IP), and the co-purified proteins were analyzed by tandem mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:10:52.992.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gary Thomas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-26 21:14:21	ID requested
⏵ 1	2026-04-05 16:10:54	announced

Publication List

Yang Y, Thomas L, Chen K, Villar-Pazos S, Haffey WD, D'Agostino A, Fanelli K, Choi YJ, Rathi V, Matlapudi MS, Greis KD, Thomas G, PACS1 syndrome mutation disrupts dynein-mediated cargo transport via HDAC6 and BICD2. Commun Biol, 9(1):(2026) [pubmed]
10.1038/s42003-026-09924-0;

Yang Y, Thomas L, Chen K, Villar-Pazos S, Haffey WD, D'Agostino A, Fanelli K, Choi YJ, Rathi V, Matlapudi MS, Greis KD, Thomas G, PACS1 syndrome mutation disrupts dynein-mediated cargo transport via HDAC6 and BICD2. Commun Biol, 9(1):(2026) [pubmed]

10.1038/s42003-026-09924-0;

Keyword List

submitter keyword: PACS1, PACS1 syndrome

submitter keyword: PACS1, PACS1 syndrome

Contact List

Gary Thomas
contact affiliation	University of Pittsburgh
contact email	thomasg@pitt.edu
lab head
Gary Thomas
contact affiliation	University of Pittsburgh
contact email	thomasg@pitt.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074970
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074970

PRIDE project URI

Repository Record List

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