PXD074932

PXD074932 is an original dataset announced via ProteomeXchange.

Title	Beyond envenomation: functional dissection of Podalia orsilochus venom uncovers a procoagulant protein and additional toxic activities
Description	Envenomation by urticating caterpillars of the family Megalopygidae is an underestimated public health issue in South America, with Podalia orsilochus being the main species implicated in cases reported in northeastern Argentina. This study aimed to characterize the toxicological properties of its venom, focusing on hematological and hemostatic effects. Venom extracts were analyzed using electrophoresis, zymography, hemolysis and coagulation assays, and chromatographic fractionation followed by immunodetection. Electrophoretic profiles revealed predominant proteins in the 10–37 kDa range, with several gelatinolytic bands detected by zymography. The venom exhibited thrombin-like and dose-dependent procoagulant activity, although weaker than that of Lonomia obliqua. A mild, direct hemolytic effect on human erythrocytes was observed, associated with degradation of Band 3, a major membrane protein. A 33-kDa protein was purified and shown to induce procoagulant activity while cross-reacting with antibodies against Losac, a Factor X-activating protein from L. obliqua venom, indicating the presence of Losac-like toxins in P. orsilochus. In conclusion, P. orsilochus venom contains components with proteolytic, hemolytic, and coagulotoxic activities, including a Losac-like protein, expanding current knowledge of lepidopteran venoms and their potential impact on human health.
HostingRepository	PRIDE
AnnounceDate	2026-03-30
AnnouncementXML	Submission_2026-03-29_16:06:45.746.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maria Pia Valacco
SpeciesList	scientific name: Megalopygidae sp. BOLD:AAA3544; NCBI TaxID: NEWT:1142745;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-25 23:19:48	ID requested
⏵ 1	2026-03-29 16:06:46	announced

10.1016/j.toxicon.2026.109086;

Gritti MA, Mart, í, nez ME, Gonzalez KY, Lobo L, ó, pez K, Teibler GP, Santoro ML, Peichoto ME, Beyond envenomation: functional dissection of Podalia orsilochus venom uncovers a procoagulant protein and additional toxic activities. Toxicon, 276():109086(2026) [pubmed]

submitter keyword: Megalopygidae, procoagulant activity, toxins, hemolysis, Losac, proteolytic enzymes

Maria Elisa Paichoto
contact affiliation	INSTITUTO DE BIOLOGIA SUBTROPICAL - NODO PUERTO IGUAZU - CENTRO CIENTIFICO TECNOLOGICO CONICET - NORDESTE - CONSEJO NACIONAL DE INVESTIGACIONES CIENTIFICAS Y TECNICAS -
contact email	mepeichoto@yahoo.com.ar
lab head
Maria Pia Valacco
contact affiliation	University of Buenos Aires
contact email	pvalacco@qb.fcen.uba.ar
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD074932

PRIDE project URI

• PRIDE
  1. PXD074932
     1. Label: PRIDE project
     2. Name: Beyond envenomation: functional dissection of Podalia orsilochus venom uncovers a procoagulant protein and additional toxic activities