PXD074929

PXD074929 is an original dataset announced via ProteomeXchange.

Title	Molecular profiling of extracellular vesicles isolated from boar reproductive fluids
Description	Extracellular vesicles (EVs) are small membrane-bound structures that facilitate intercellular communication in the reproductive system, modulating gamete maturation, capacitation, immunomodulation, and fertilization. Despite pigs’ high relevance as a biomedical model, many aspects of EV biology remain poorly understood. EVs from boar seminal plasma (SP) are relatively well studied, whereas epididymosomes remain largely uncharacterized. Therefore, this study aimed to isolate and characterize EVs from the caput, corpus, and cauda regions of boar epididymis, as well as from SP, with their further precise analysis towards their interaction with sperm. We successfully obtained EVs from all studied fluids with sufficient purity. Importantly, our isolation protocol preserved the EVs’ ability to interact with sperm, demonstrated by staining with lipophilic dyes and biotin labeling experiments, confirming precisely their interaction and cargo transfer to sperm cells. Well-established EV markers, such as Alix and tetraspanins, were detected in the EVs, and additionally, phosphorylated, ubiquitinated, and sialylated proteins were uniquely identified. Furthermore, we employed a proteomic approach to characterize EV proteins and investigate their functional roles using the Gene Ontology (GO) database. This study contributes valuable insights into the molecular composition and functional properties of EVs from the male reproductive tract. It may provide a solid framework for further basic and translational research in reproductive biology and biomedicine.
HostingRepository	PRIDE
AnnounceDate	2026-07-20
AnnouncementXML	Submission_2026-07-19_16:11:46.041.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jan Rasl
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	timsTOF SCP

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-25 19:31:46	ID requested
⏵ 1	2026-07-19 16:11:46	announced

Kraus V, Doleckova B, Frolikova M, Sanovec O, Spevakova D, Pilsova Z, Pilsova A, Komrskova K, Simonik O, Postlerova P, Molecular Profiling of Extracellular Vesicles Isolated from Boar Reproductive Fluids. J Extracell Vesicles, 15(7):e70329(2026) [pubmed]

10.1002/jev2.70329;

submitter keyword: protein cargo, proteomics,epididymis, seminal plasma, spermatozoa, reproduction

Pavla Postlerova
contact affiliation	Laboratory of Reproductive Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec, Czech Republic
contact email	pavla.postlerova@ibt.cas.cz
lab head
Jan Rasl
contact affiliation	Intitute of Biotechnology
contact email	jan.rasl@ibt.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD074929

PRIDE project URI

• PRIDE
  1. PXD074929
     1. Label: PRIDE project
     2. Name: Molecular profiling of extracellular vesicles isolated from boar reproductive fluids