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PXD074916
PXD074916 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Based on Mass Spectrometry Reveals MEGF8 as a Potential Biomarker for Diabetes |
| Description | Diabetes mellitus constitutes a major global health challenge. Current diagnostic markers, such as fasting glucose and glycated hemoglobin A1c (HbA1c), show limitations in accuracy and sensitivity in epidemiological settings. This study aimed to identify novel serum biomarkers for diabetes using a multi-stage approach encompassing discovery, verification, and clinical correlation. In the discovery phase, data-independent acquisition mass spectrometry (DIA-MS) analysis of serum from 107 individuals (53 controls, 54 diabetic subjects) identified 345 differentially expressed proteins (DEPs) after filtering. Receiver operating characteristic (ROC) analysis of these DEPs highlighted five proteins—MEGF8, PLXNB2, SHBG, CLEC3B, and GSN—with high diagnostic potential (AUC > 0.85). Subsequent parallel reaction monitoring (PRM) verification in an independent cohort (46 diabetic vs. 46 control samples) confirmed consistent expression trends for four of these candidates (MEGF8, PLXNB2, SHBG, and GSN), with AUCs of 0.782, 0.763, 0.845, and 0.671, respectively. Further validation by ELISA in a clinical cohort (43 diabetic vs. 31 control subjects) demonstrated significant upregulation of MEGF8 in diabetic serum (1081.00 ± 250.49 pg/mL vs. 950.30 ± 189.64 pg/mL; P = 0.0187). Correlation analysis within this cohort revealed that serum MEGF8 levels were specifically and positively associated with key glycemic and insulin resistance indices, including fasting blood glucose (r = 0.268, P = 0.021), HbA1c (r = 0.295, P = 0.011), and HOMA-IR (r = 0.350, P = 0.002), but not with adiposity measures or lipid profiles. Collectively, through multi-stage validation, MEGF8 emerges as a promising and specific serum biomarker closely linked to hyperglycemia and insulin resistance in diabetes. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-25 |
| AnnouncementXML | Submission_2026-02-25_16:40:21.979.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Liru Feng |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-25 16:39:02 | ID requested | |
| ⏵ 1 | 2026-02-25 16:40:22 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Diabetes, Serum, Biomarker, MEGF8, proteomics, DIA-MS, PRM |
Contact List
| Wenxu Hong | |
|---|---|
| contact affiliation | Shenzhen Center for Chronic Disease Control, Shenzhen Dermatology Hospital; Shenzhen Institute of Dermatology |
| contact email | szbloodcenter@hotmail.com |
| lab head | |
| Liru Feng | |
| contact affiliation | Shenzhen Center for Chronic Disease Control, Shenzhen Dermatology Hospital; Shenzhen Institute of Dermatology |
| contact email | jqryy@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
