PXD074883

PXD074883 is an original dataset announced via ProteomeXchange.

Title	Serum Proteomics Unveils Diagnostic and Stratifying Biomarkers and Underlying Mechanisms in Cerebral Microbleeds
Description	Cerebral microbleeds (CMBs) are small brain vascular lesions detectable on magnetic resonance imaging and associated with elevated risks of stroke and cognitive decline. This study aimed to identify circulating protein biomarkers for early CMB detection and to elucidate molecular mechanisms across CMB subtypes. Serum from 43 patients with MRI-confirmed CMBs and 38 healthy controls was analysed using high performance liquid chromatography combined with liquid chromatography tandem mass spectrometry. We identified 151 differentially expressed proteins, and enrichment analyses highlighted inflammation, extracellular matrix remodelling, and lipid metabolism. Subtype-stratified enrichment further implicated MAPK/RAF/ERK signalling in deep lesions and insulin-like growth factor signalling in lobar lesions. Machine learning was used for candidate selection, and six proteins were prioritised, including matrix metallopeptidase 3 (MMP3), epidermal growth factor containing fibulin-like extracellular matrix protein 1 (EFEMP1), tissue inhibitor of metalloproteinases 1 (TIMP1), uromodulin (UMOD), nectin cell adhesion molecule 1 (NECTIN1), and ubiquitin A52 ribosomal fusion protein 1 (UBA52). Validation by enzyme-linked immunosorbent assay confirmed robust diagnostic performance for MMP3, EFEMP1, TIMP1, and UMOD with area under the curve values above 0.7, and EFEMP1 correlated positively with CMB burden. Western blotting further verified subtype-specific expression patterns and identified reticulocalbin 1 (RCN1), neogenin 1 (NEO1), and amyloid precursor-like protein 1 (APLP1) as discriminators between lobar and deep CMBs, with area under the curve values above 0.8. These findings define a serum proteomic landscape for CMBs, provide mechanistic insight into subtype-specific pathways, and support the development of non-invasive biomarkers for early diagnosis and subtype differentiation.
HostingRepository	iProX
AnnounceDate	2026-02-21
AnnouncementXML	Submission_2026-02-25_00:51:32.699.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wumeng Yin
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-25 00:50:00	ID requested
⏵ 1	2026-02-25 00:51:33	announced

Dataset with its publication pending

submitter keyword: Cerebral microbleeds, Serum proteomics, Biomarkers, MAPK/RAF/ERK signalling, IGF signalling

Yuming Xu
contact affiliation	NHC Key Laboratory of Prevention and Treatment of Cerebrovascular Diseases
contact email	xuyuming@zzu.edu.cn
lab head
Wumeng Yin
contact affiliation	The First Affiliated Hospital of Zhengzhou University
contact email	ywm020323@163.com
dataset submitter

iProX dataset URI