PXD074882

PXD074882 is an original dataset announced via ProteomeXchange.

Title	Global profiling of myristoylation in human lung cancer cells
Description	The experimental design included Calu-1 and H460 cells subjected to four treatment groups (Myr, YnMyr, YnMyr + 10 nM IMP-1088, YnMyr + 100 nM IMP-1088), each with four biological replicates. All samples were ligated to AzDB and affinity enriched. Following digestion, all peptides were subjected to LC-MS/MS analysis and quantified by LFQ. For the AzDB reagent, LysC and trypsin digestion first release unmodified peptides(other peptides from myristoylated proteins or background peptides). Subsequently, myristoylated peptides remaining on the agarose resin are released using formic acid, allowing the two peptide fractions to be detected separately.
HostingRepository	iProX
AnnounceDate	2026-02-21
AnnouncementXML	Submission_2026-02-25_00:48:45.122.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yikun Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-25 00:48:16	ID requested
⏵ 1	2026-02-25 00:48:45	announced

Dataset with its publication pending

submitter keyword: N-myristoylation, click chemistry, CuAAC, DADPS Azide Biotin

Xiao Zhang
contact affiliation	Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine
contact email	728002276@shsmu.edu.cn
lab head
Yikun Wang
contact affiliation	Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine
contact email	823939160@qq.com
dataset submitter

iProX dataset URI