⮝ Full datasets listing
PXD074878
PXD074878 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative TMT phosphoproteomics in neonatal mouse ventricular myocytes (NMVMs) upon NUAK1 overexpression |
| Description | This dataset contains TMT-based quantitative phosphoproteomics data generated from neonatal mouse ventricular myocytes (NMVMs) comparing control cells versus NUAK1 overexpression (three biological replicates per group; CTL_1–3 and OE_1–3). Samples were analyzed by LC–MS/MS with fractionated runs (raw fractions F1–F3). MS/MS spectra were searched using MaxQuant (v1.6.15.0) against the UniProt Mus musculus reference proteome (release 2024-06-04). Enzyme was set to Trypsin/P. Fixed modification: Carbamidomethyl (C). Variable modifications: Oxidation (M), Protein N-term acetylation, and Phospho (STY). Mass tolerances were set to 20 ppm (first search) and 5 ppm (main search) for precursor ions and 20 ppm for fragment ions. Protein and PSM FDR were controlled at 1%. The submission includes the raw files and identification/quantification summary tables used for downstream QC and differential phosphorylation analyses. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-25 |
| AnnouncementXML | Submission_2026-07-01_00:54:23.876.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yangjinming Bai |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | phosphorylated residue |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-25 00:40:09 | ID requested | |
| ⏵ 1 | 2026-07-01 00:54:24 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: NMVM, neonatal mouse ventricular myocytes, NUAK1, overexpression, TMT, phosphoproteomics, phosphorylation, LC-MS/MS, MaxQuant, Mus musculus |
Contact List
| Yi-Gang Li | |
|---|---|
| contact affiliation | Department of Cardiology, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China |
| contact email | liyigang@xinhuamed.com.cn |
| lab head | |
| Yangjinming Bai | |
| contact affiliation | Department of Cardiology, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China |
| contact email | byjm980604@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
