PXD074839

PXD074839 is an original dataset announced via ProteomeXchange.

Title	Lignin and protein profiles of animal feed coproducts generated in a dry grind corn ethanol biorefinery
Description	Corn bioethanol production generates large volumes of animal feed coproducts with nutritional value largely determined by their fiber and protein content. Here, we combined microscopic and biochemical lignin quantification, and proteomic analysis to characterize the corn flour (CF) feedstock and downstream dried distiller’s grains with solubles (DDGS) and corn fermented protein (CFP) fractions from an industrial bioethanol plant. We show that the industrial processing concentrates lignin up to 8% of the dry weight of distiller grain fractions, representing an opportunity for its recovery and utilization. We also characterized the proteome of these materials, identifying ~4,200 maize (Zea mays) proteins, and ~300 yeast (Saccharomyces cerevisiae) and ~90 bacterial (Escherichia coli) proteins. Interestingly, 90% of the maize proteins present in the CF feedstock were detected in the DDGS and CFP fractions, suggesting mild protein breakdown during processing. Proteomics analysis enabled us to map the abundance levels of specific enzymes involved in lignin and starch biosynthesis in the maize endosperm, and key yeast enzymes involved in the glycolysis and fermentation pathways, revealing limiting enzymatic steps in these metabolic pathways. Additionally, we found that seed storage proteins, particularly globulins and oleosins, were enriched in the high-protein CFP fraction, consistent with its higher nutritional value. Microbial proteomics revealed that S. cerevisiae and E. coli each contributed ~3% of the total protein present in DDGS and CFP fractions. This study provides the first proteomic analysis of corn ethanol coproducts and identifies targets to enhance their utilization via maize genetics, microbial engineering, or lignin valorization strategies.
HostingRepository	PRIDE
AnnounceDate	2026-08-25
AnnouncementXML	Submission_2026-08-25_07:40:18.775.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Noah Davis
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: NEWT:4577; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: NEWT:4932; scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-24 21:17:24	ID requested
⏵ 1	2026-08-25 07:40:19	announced

10.1186/S13068-026-02812-Z;

submitter keyword: Escherichia coli,Zea mays, Saccharomyces cerevisiae, LC-MS/MS

Jaime Antonio Barros-Rios
contact affiliation	Division of Plant Science and Technology, College of Agriculture Food and Natural Resources and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA
contact email	jaime.barros@missouri.edu
lab head
Noah Davis
contact affiliation	University of Missouri
contact email	davisnoahn@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074839
     1. Label: PRIDE project
     2. Name: Lignin and protein profiles of animal feed coproducts generated in a dry grind corn ethanol biorefinery