PXD074801

PXD074801 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics profiling reveals the potential regulating target for intervening muscle softening of shrimp L. vannamei during refrigeration
Description	Herein, to unveil the role of phosphorylation modifications in texture softening of shrimp muscle during refrigeration, the phosphorylation profiling of muscle structural proteins after chilling storage at 4 oC for 120h was comparatively investigated by phosphoproteomics. Totally, the unique 1255 differential abundance phosphopeptides (DAPPs), encompassing 2128 phosphorylated sites in 608 phosphoproteins, were identified and they were primarily classified into intracellular myofibril, cytoskeleton, and assembly regulating factors. GO and KEEG annotation analysis revealed that these DAPPs were dominantly enriched in ultrastructure assembly and myofilament contraction of sarcomere architecture, pathways of mTOR signaling, autophagy, and energy metabolism. Meanwhile, the profiling of concomitant dephosphorylation of kinases, phosphatases, and assembly regulating factors revealed the disassembly potential of sarcomeric architecture through SP and R/KxxS motifs. Moreover, the predominate nodes in protein-protein interaction (PPI) network included mitogen-activated protein kinase 14, ubiquitin-ribosomal protein eL40 fusion protein, cAMP-dependent protein kinase catalytic subunit, heat shock protein, and myosin light chain kinase; meanwhile, these proteins showed significantly high correlation with texture deterioration, indicating their potential as target regulators for intervening the muscle softening process. Collectively, this study, for the first time, deciphered the undefined mechanisms governing muscle softening of shrimp L. vannamei during refrigeration from the phosphorylation-dependent perspective. Furthermore, these insights might provide valuable alternatives for developing target strategies against muscle softening by manipulating phosphoproteins.
HostingRepository	iProX
AnnounceDate	2026-02-12
AnnouncementXML	Submission_2026-02-24_16:52:29.810.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Defeng Xu
SpeciesList	scientific name: Penaeus vannamei nodavirus; NCBI TaxID: 430911;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-24 16:52:08	ID requested
⏵ 1	2026-02-24 16:52:30	announced

Dataset with its publication pending

submitter keyword: shrimp, texture softening, phosphoproteomic profiling, structural proteins

Defeng Xu
contact affiliation	Guangdong Medical University
contact email	13827198525@163.com
lab head
Defeng Xu
contact affiliation	Guangdong Medical University
contact email	13827198525@163.com
dataset submitter

iProX dataset URI