PXD074771

PXD074771 is an original dataset announced via ProteomeXchange.

Title	LCMS spermatozoa and seminal plasma
Description	This dataset contains raw and processed LC–MS/MS data from a paired proteomic analysis of seminal plasma and spermatozoa fractions obtained from the same ejaculates of two adult stallions. Samples were analyzed using a Vanquish nanoLC system coupled to an Orbitrap Exploris 240 mass spectrometer operated in data-dependent acquisition (DDA) mode. Database searches were performed in Proteome Discoverer (v3.1) using the Sequest HT search engine against the Equus caballus UniProt reference proteome (UP000002281). Peptide and protein identifications were filtered at 1% FDR with a minimum of two unique peptides per protein. The dataset supports abundance-based comparison of functional specialization between seminal plasma and spermatozoa compartments.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_06:18:17.279.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD074771
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Grzegorz Smolucha
SpeciesList	scientific name: Equus caballus (Horse); NCBI TaxID: NEWT:9796;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-24 09:54:43	ID requested
⏵ 1	2026-05-21 06:18:17	announced

10.6019/PXD074771;

submitter keyword: seminal plasma, exploris240,ecuus qabalus, spermatozoa

Grzegorz Smolucha
contact affiliation	Department of Animal Molecular Biology, Laboratory of Proteomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
contact email	grzegorz.smolucha@iz.edu.pl
lab head
Grzegorz Smolucha
contact affiliation	National Research Institute of Animal Profuctio, Laboratory of Proteomics
contact email	grzegorz.smolucha@iz.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074771

PRIDE project URI

• PRIDE
  1. PXD074771
     1. Label: PRIDE project
     2. Name: LCMS spermatozoa and seminal plasma