PXD074740

PXD074740 is an original dataset announced via ProteomeXchange.

Title	Triptolide clears Staphylococcus aureus infection by targeting XIAP to induce host apoptosis while preserving gut microbiota homeostasis [Mouse lung macrophages]
Description	Staphylococcus aureus (SA) remains a global health threat due to its increasing drug resistance and intracellular persistence, which compromise the efficacy of conventional antibiotics. Host-directed therapy (HDT) has emerged as a promising alternative by modulating host immunity. With the multi-targeting and immunomodulatory properties, Traditional Chinese Medicine (TCM) monomers represent ideal candidates for HDT. However, their ability to promote host immunity-mediated SA clearance remains largely unexplored. Here, we identified triptolide as a compound that facilitates intracellular clearance of SA and MRSA. Mechanistic studies revealed that triptolide directly binds to the X‑linked inhibitor of apoptosis protein (XIAP) to inhibit XIAP-caspase interaction, thereby releasing caspase inhibition and inducing apoptosis. Moreover, treatment with triptolide reduced bacterial loads and alleviated lung inflammation in mice infected with SA and MRSA, concurrently maintaining microbiota homeostasis and improving metabolic function. Taken together, these findings establish triptolide as a promising lead compound for the development of HDT against SA and MRSA infections.
HostingRepository	PRIDE
AnnounceDate	2026-05-18
AnnouncementXML	Submission_2026-05-18_05:21:26.460.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhe Lu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-23 09:41:24	ID requested
⏵ 1	2026-05-18 05:21:26	announced

Dataset with its publication pending

submitter keyword: triptolide, microbiota homeostasis, host-directed therapy, methicillin-resistant Staphylococcus aureus, apoptosis, XIAP,Staphylococcus aureus

Cui Hua Liu
contact affiliation	Institute of Microbiology
contact email	Lu1457883143@gmail.com
lab head
Zhe Lu
contact affiliation	Institute of Microbiology, Beijing
contact email	lu1457883143@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074740
     1. Label: PRIDE project
     2. Name: Triptolide clears Staphylococcus aureus infection by targeting XIAP to induce host apoptosis while preserving gut microbiota homeostasis [Mouse lung macrophages]