⮝ Full datasets listing
PXD074727
PXD074727 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Narrow Developmental Window Defines PKN2's Essential Role in Ventricular Chamber Morphogenesis |
| Description | Protein kinase N2 (PKN2) is essential for embryonic heart development, but the precise timing and cellular requirement in cardiomyocytes are not well understood. In a constitutive cardiomyocyte specific knockout (cKO) of PKN2, we observed partially penetrant early postnatal lethality, nonprogressive systolic dysfunction in survivors, and a distinctive coin pouch ventricular geometry resembling the embryonic shape, linking the phenotype to a developmental origin rather than ongoing pathology. By tracking heart morphogenesis, we found that mutant and control hearts were still grossly indistinguishable at E10.5, defining an unbiased stage for molecular profiling before secondary remodeling. Integrative RNAseq, proteomics, and phosphoproteomics at E10.5 revealed concurrent induction of actin cytoskeleton and motility programs and repression of chromosome condensation and mitotic modules in PKN2 cKO hearts. These molecular changes coincided with reduced cardiomyocyte proliferation at E10.5 E11.5 and early divergence in ventricular shape trajectories. Inducible cardiomyocyte specific PKN2 knockout hearts analyzed by quantitative light-sheet microscopy based morphometrics pinpointed the critical dependence window: recombination induced at E7.5 reproduced the ventricular geometry characteristic of constitutive PKN2 cKO hearts, whereas induction at E10.5 spared gross morphology. Together, these data show that cardiomyocytes depend on PKN2 during an early proliferative and migratory phase that drives ventricular wall formation, after which PKN2 becomes dispensable for heart morphogenesis. This temporally restricted requirement reconciles previous observations of PKN2s essential role in mesodermal and epithelial morphogenesis, suggesting a shared function in coordinating cytoskeletal remodeling with tissue expansion during organogenesis. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-05-05 |
| AnnouncementXML | Submission_2026-05-05_21:06:46.344.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alexandre Rosa Campos |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Phospho; Oxidation |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-22 23:27:56 | ID requested | |
| ⏵ 1 | 2026-05-05 21:06:47 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: PKN2, cardiac development, mouse models, DatasetType:Proteomics |
Contact List
| Ju Chen | |
|---|---|
| contact affiliation | Department of Medicine, UCSD |
| contact email | juchen@health.ucsd.edu |
| lab head | |
| Alexandre Rosa Campos | |
| contact affiliation | SBP Medical Discovery Institute |
| contact email | arosacampos@sbpdiscovery.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100933/ |
