PXD074718 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Quantitative analysis of lipoproteins from Haloferax volcanii using aliA and aliB deletion mutants |
| Description | This project aimed to analyze protein lipidation, a widespread strategy for anchoring proteins to cellular membranes across all domains of life, in the model archaeon Haloferax volcanii. Using deletion mutants of aliA and aliB, the first archaeal enzymes shown to be involved in lipobox-containing protein biogenesis, we employed large-scale Triton X-114 fractionation followed by quantitative proteomics to systematically analyze AliA- and AliB-dependent lipoprotein lipidation. Deletion of aliA affected most predicted lipoproteins in Hfx. volcanii, markedly diminishing their TX-114 enrichment, indicating reduced hydrophobicity. This establishes AliA as the primary enzyme responsible for archaeal lipoprotein lipidation. In contrast, deletion of aliB affected only a small subset of predicted lipoproteins. In addition to defining distinct and non-redundant roles for AliA and AliB, this study provides the first large-scale experimental validation of predicted archaeal lipoproteins and identifies candidate components of the archaeal lipoprotein biogenesis pathway. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-18 |
| AnnouncementXML | Submission_2026-08-18_11:56:59.401.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Stefan Schulze |
| SpeciesList | scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: NEWT:2246; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-21 15:58:04 | ID requested | |
| ⏵ 1 | 2026-08-18 11:56:59 | announced | |
Publication List
Keyword List
| submitter keyword: Haloferax volcanii, archaea, Triton X-114, lipoproteins |
Contact List
| Mechthild Pohlschroder |
|---|
| contact affiliation | University of Pennsylvania, Department of Biology |
| contact email | pohlschr@sas.upenn.edu |
| lab head | |
| Stefan Schulze |
|---|
| contact affiliation | Rochester Institute of Technology |
| contact email | sschulze@sas.upenn.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD074718 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074718
- Label: PRIDE project
- Name: Quantitative analysis of lipoproteins from Haloferax volcanii using aliA and aliB deletion mutants
