PXD074714

PXD074714 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic profiling reveals viral proteins and associated host proteomic alterations in glioblastoma
Description	Glioblastoma (GB) is a WHO grade 4 brain cancer with dismal prognosis, yet its aetiology remains poorly defined. Although viral involvement has been proposed, findings across studies remain inconsistent, reflecting inherent limitations of individual technologies and cohort size. Here we applied metaproteomic profiling to a publicly available GB proteome dataset (12 control, 21 adjacent, 159 tumour) and an independent cohort of 81 samples (37 control, 44 tumour) to detect viral proteins in tumour and controls tissues. Across cohorts, we detected viral proteins from diverse species, with human herpesviruses (HHV-1, 2, and 8) more frequently detected in GB tumours compared with control tissues. Analysis of the host tumour proteome revealed differential abundance of proteins related to transcriptional regulation, RNA processing, protein translation, immune responses, and mitochondrial-associated metabolism. Correlation analysis identified associations between viral and human proteins, with several linked to biological processes previously implicated in DNA virus-host interactions. Further stratification of tumour by HHV-1 status showed consistent alterations in proteins associated with mitochondrial-associated metabolism, protein turnover, and cell adhesion/signalling. In summary, this study demonstrates the feasibility of metaproteomics for detecting viral components in archival GB tissues. Using this approach, we observed differences in viral protein landscape across cohorts and identified associations between viral presence and host proteomic features, providing a protein-level framework for future studies of virus-host interactions in GB.
HostingRepository	PRIDE
AnnounceDate	2026-03-11
AnnouncementXML	Submission_2026-03-11_02:00:06.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Bavani Gunasegaran
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-21 10:37:54	ID requested
⏵ 1	2026-03-11 02:00:06	announced

Dataset with its publication pending

submitter keyword: Human, LC-MSMS, Brain, Tissues, Glioblastoma

Dr Benjamin Heng
contact affiliation	1: Macquarie Medical School, Faculty of Medicine, Health and Human Sciences, Macquarie University, NSW, Australia 2: School of Medical Sciences, Faculty of Medicine and Health, University of Sydney, NSW, Australia
contact email	benjamin.heng@sydney.edu.au
lab head
Bavani Gunasegaran
contact affiliation	Macquarie University
contact email	bavaniguna@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD074714

PRIDE project URI

• PRIDE
  1. PXD074714
     1. Label: PRIDE project
     2. Name: Metaproteomic profiling reveals viral proteins and associated host proteomic alterations in glioblastoma