PXD074698

PXD074698 is an original dataset announced via ProteomeXchange.

Title	HYDIN2 knockdown in NT2 cells analyses by LFQ
Description	Segmental duplications at proximal loci can generate transcriptionally active chimeric pseudogenes (φgenes) by fusing contiguous sequences from two parental genes. These chimeric φgenes often co-opt 5′ regulatory elements for activation and acquire 3′ modifications that enhance transcript stability, with some retaining coding potential to produce novel or truncated proteins. Functional analyses indicate that such φgenes contribute to human-specific regulatory processes; notably, HYDIN2 knockdown impairs neuronal differentiation. Proteomic profiling of HYDIN2 knockdown cells was performed to identify downstream effectors and pathways associated with its regulatory function.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:14:03.741.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yash Kuber
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-20 15:42:58	ID requested
⏵ 1	2026-05-24 16:14:04	announced

10.1093/nar/gkag375;

Jadhav AR, Sahoo S, Kuber YL, Hatwar K, Varanwal A, Tanwar K, Palwankar S, Chatrasal G, Mali AM, Bapat SA, genes) contribute to genetic and functional diversity of the human genome. Nucleic Acids Res, 54(8):(2026) [pubmed]

submitter keyword: HYDIN2, Chimeric pseudogene, LFQ proteomics

Sharmila A. Bapat
contact affiliation	National centre for cell science, Pune, India
contact email	sabapat@nccs.res.in
lab head
Yash Kuber
contact affiliation	National centre for cell science, Pune, India
contact email	kuberyash21@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074698

PRIDE project URI

• PRIDE
  1. PXD074698
     1. Label: PRIDE project
     2. Name: HYDIN2 knockdown in NT2 cells analyses by LFQ