⮝ Full datasets listing
PXD074693
PXD074693 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | 2-O-methylation Maintains Ribosome Structural and Translation Integrity |
| Description | In eukaryotic ribosomes, ~2% of RNA nucleotides undergo 2'-O-methylation, a conserved cellular mechanism thought to be critical for maintaining and regulating translation. Here, we use ribosome profiling, translation assays, proteomics, and high-resolution structural analyses to show that loss of native 2'-O-methylation in yeast ribosomes disproportionally affect the translation of ribosomal protein transcripts, driven by changes in codon usage and recognition of structured RNA. Translation reprograming by the hypomethylated ribosomes is supported by reduced thermostability and high-resolution evidence of altered ribosomal structures and conformations. Consistent with the roles of the affected proteins in sustaining cellular fitness, hypomethylated ribosomes under stress exhibit selective loss of downregulated proteins and misassembly associated with methylation loss. Our data provide structural and mechanistic insights into how 2'-O-methylation supports ribosome integrity and mediates cellular stress responses. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-20 |
| AnnouncementXML | Submission_2026-02-20_12:47:57.170.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Molly Hopper |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | Acetyl; Carbamidomethyl; GG; Oxidation |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-20 12:13:56 | ID requested | |
| ⏵ 1 | 2026-02-20 12:47:58 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: ribosomal RNA modification, fibrillarin, DatasetType:Proteomics |
Contact List
| Hong Li | |
|---|---|
| contact affiliation | Van Andel Institute |
| contact email | hong.li@vai.org |
| lab head | |
| Molly Hopper | |
| contact affiliation | Van Andel Institute |
| contact email | molly.hopper@vai.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100922/ |
