PXD074668

PXD074668 is an original dataset announced via ProteomeXchange.

Title	Nitrate-responsive Sialin2 Couples Stress Granule Assembly and Clearance to Maintain Neuronal Homeostasis
Description	Stress granules (SGs) are phase-separated ribonucleoprotein condensates that support cellular adaptation to acute stress, but how extracellular signals coordinate their assembly and recovery-phase clearance remains unclear. Here, we show that nitrate-responsive Sialin2 coordinates SG homeostasis across the stress cycle. During acute stress, Sialin2 undergoes liquid-liquid phase separation and co-assembles with G3BP-centered scaffolds to promote SG formation. During recovery, Sialin2 preserves liquid-like SG dynamics and promotes autophagy-linked clearance. Sialin2 loss delays SG resolution, promotes retention of ALS-linked FUS and TDP-43 variants, and enhances APP-related amyloidogenic output. In an ALS mouse model, nitrate treatment reduces SG-like pathology and neuroinflammation, preserves neuronal integrity, and delays functional decline. Together, these findings define a nitrate-Sialin2 signaling module that couples SG assembly to recovery-phase clearance and links inorganic anion sensing to protection against neurodegeneration.
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-03_09:07:55.995.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyu Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-19 21:35:06	ID requested
⏵ 1	2026-09-03 09:07:56	announced

Dataset with its publication pending

submitter keyword: Nitrate

Sialin2

Stress granules

Liquid-liquid phase separation

Autophagy

Neurodegeneration

Xiaoyu Li
contact affiliation	Beijing, You An Men Wai Xi Tou Tiao No.10, Capital Medical University, Beijing Laboratory of Oral Health
contact email	xiaoyu9449@ccmu.edu.cn
lab head
Xiaoyu Li
contact affiliation	Capital Medical University
contact email	xiaoyu9449@ccmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD074668

PRIDE project URI

• PRIDE
  1. PXD074668
     1. Label: PRIDE project
     2. Name: Nitrate-responsive Sialin2 Couples Stress Granule Assembly and Clearance to Maintain Neuronal Homeostasis