PXD074614

PXD074614 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling to uncover developmental dynamics of autophagy in the cortex
Description	Autophagy is an evolutionarily conserved degradation and recycling process through which cells deliver cytoplasmic components such as toxic or defective proteins and organelles to lysosomes for clearance. Unlike dividing cells, neurons depend on degradative pathways to prevent the build-up of cellular waste and to sustain nutrient and energy homeostasis. Emerging evidence indicates that autophagy is particularly critical during early development when neuronal circuits are being established, synaptic connections refined, and activity-dependent mechanisms sculpt overall network architecture. Accordingly, loss of key autophagy-related genes in newly formed neurons disrupts differentiation, synaptic formation and neurotransmission. Despite these insights, the developmental regulation of autophagy genes remains poorly understood, and the composition of the autophagic machinery at synapses is still largely unresolved. To address this, we examined the autophagy-associated proteome within synaptosomes to better understand how autophagic proteins contribute to synaptic processes during critical stages of network formation. Along with transcriptomics data, we provide a foundation for identifying therapeutic targets for neurological disorders linked to impaired synaptic and cellular homeostasis.
HostingRepository	PRIDE
AnnounceDate	2026-04-03
AnnouncementXML	Submission_2026-04-03_04:00:55.905.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD074614
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Annapaola Andolfo
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-18 18:14:29	ID requested
⏵ 1	2026-04-03 04:00:56	announced

10.6019/PXD074614;

submitter keyword: autophagy, synapsis, synaptosomes,Proteomics, murine cortical development

Palmieri Michela
contact affiliation	Rett Research Unit, Division of Neuroscience IRCCS San Raffaele Scientific Institute Via Olgettina, 60 20132 Milan, Italy
contact email	palmieri.michela@hsr.it
lab head
Annapaola Andolfo
contact affiliation	Ospedale San Raffaele
contact email	andolfo.annapaola@hsr.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074614
     1. Label: PRIDE project
     2. Name: Proteomic profiling to uncover developmental dynamics of autophagy in the cortex