PXD074603

PXD074603 is an original dataset announced via ProteomeXchange.

Title	A porcine spectral assay library to quantify brain proteome by DIA-MS
Description	The peptide-centric analysis requires spectral assay libraries that allow for the extraction of quantitative data in mass spectrometry-based methods, including data-independent acquisition (DIA), parallel reaction monitoring (PRM), or selected reaction monitoring (SRM). Such spectral libraries have been generated for humans (pan-human, T-cells, HLA subtypes) and other organisms such as mouse, E. coli, zebrafish, S. aureus, M. tuberculosis, etc. Here, we focused on the pig (Sus scrofa), a biomedical model capable of recapitulating the complexity of human pathology. We analyzed brain tissues and cerebrospinal fluids from a transgenic minipig model of Huntington's disease subjected to multiple extraction and fractionation steps. We generated a porcine spectral library for 8,321 proteins and demonstrated that this library provides a valuable resource to confidently and reproducibly quantify porcine proteins at the proteome-wide level. Via targeted assays, it enables the quantification of selected proteins with a key role not only in neuroscience.
HostingRepository	PRIDE
AnnounceDate	2026-05-18
AnnouncementXML	Submission_2026-05-18_11:57:59.973.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jakub Cervenka
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-18 12:17:52	ID requested
⏵ 1	2026-05-18 11:58:00	announced

Dataset with its publication pending

submitter keyword: None

Petr Vodicka
contact affiliation	Laboratory of Applied Proteome Analyses, Institute of Animal Physiology and Genetics of the Czech Academy of Sciences, Libechov, Czech Republic
contact email	vodicka@iapg.cas.cz
lab head
Jakub Cervenka
contact affiliation	Laboratory of Proteomics, Institute of Biochemistry and Experimental Oncology, First Faculty of Medicine, Charles University, Prague, Czech Republic
contact email	jakub.cervenka@lf1.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074603

PRIDE project URI

• PRIDE
  1. PXD074603
     1. Label: PRIDE project
     2. Name: A porcine spectral assay library to quantify brain proteome by DIA-MS