PXD074583

PXD074583 is an original dataset announced via ProteomeXchange.

Title	Phosphoethanolamine modification to lipid A found in five distinct Akkermansia species
Description	We investigated how lipid A is remodeled across the Akkermansia genus, focusing on whether structurally distinct species share common modifications and how these changes relate to antimicrobial peptide resistance. Lipid A was extracted from representative isolates spanning five Akkermansia species (A. muciniphila, A. massiliensis, A. biwaensis, A. ignis, and A. durhamii; total N=55) and profiled by negative-ion MALDI mass spectrometry using an on-target FLAT workflow. Across all species, we detected lipid A species bearing a +123 Da mass shift consistent with phosphoethanolamine (pEtN) addition, and confirmed the modification by tandem MS on dominant modified and unmodified lipid A ions. We quantified isolate-to-isolate variation in the relative abundance of pEtN-modified versus unmodified lipid A and tested functional relevance by measuring colistin susceptibility in representative strains. Higher pEtN modification ratios correlated with decreased colistin susceptibility. We further assessed regulation of this remodeling by sampling across growth phases and found that pEtN-modified lipid A increased as cultures entered stationary phase, independent of colistin exposure. Finally, we used label-free proteomics to identify cell-envelope and envelope biogenesis-associated proteins that change with growth stage in strains that do versus do not upregulate pEtN-modified lipid A, providing candidate pathways linked to outer membrane remodeling. Overall, this work reports a conserved pEtN lipid A modification across diverse Akkermansia species and connects its relative abundance to antimicrobial susceptibility and growth-phase-dependent regulation, laying groundwork for future studies of species-specific host–microbe interactions in the gut.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-27_10:21:24.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Silas Porter Crenna
SpeciesList	scientific name: Akkermansia muciniphila; NCBI TaxID: NEWT:239935;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-18 00:50:39	ID requested
⏵ 1	2026-04-27 10:21:24	announced

Dataset with its publication pending

submitter keyword: Phosphoethanolamine, Akkermansia muciniphila, Lipid A,Akkermansia, pEtN

Helena Pětrošová
contact affiliation	Group Leader, Mass Spectrometry Imaging, Department of Biochemistry and Microbiology, University of Victoria Genome BC Proteomics Centre, Adjunct Assistant Professor, University of Victoria
contact email	hpetros@uvic.ca
lab head
Silas Porter Crenna
contact affiliation	University of Victoria
contact email	sportercrenna@uvic.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074583
     1. Label: PRIDE project
     2. Name: Phosphoethanolamine modification to lipid A found in five distinct Akkermansia species