PXD074569

PXD074569 is an original dataset announced via ProteomeXchange.

Title	Glycoproteomic and genetic analysis of N-glycosylation of complement component C3 reveals immune pathway regulation
Description	This dataset comprises site-specific glycopeptide analysis of complement component C3 from plasma samples of 816 adults from the Korčula cohort (Croatia). C3 glycopeptides were enriched using Concanavalin A lectin affinity chromatography and analyzed by nano-LC-MS/MS. The study characterizes the N-glycan repertoire at both occupied glycosylation sites (N63 and N917), which carry exclusively high-mannose structures including unusual monoglucosylated glycoforms (Glc1Man9GlcNAc2, Glc1Man8GlcNAc2). Glycopeptide quantification was performed to assess associations with demographic and biochemical traits, and to conduct genome-wide association analysis identifying genetic determinants of C3 glycosylation. This represents the first large-scale population-level analysis of site-specific C3 N-glycosylation.
HostingRepository	PRIDE
AnnounceDate	2026-05-30
AnnouncementXML	Submission_2026-05-30_14:08:47.712.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dinko Šoić
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-17 17:29:01	ID requested
⏵ 1	2026-05-30 14:08:48	announced

Dataset with its publication pending

submitter keyword: glycoproteomics,complement C3, high-mannose glycans, N-glycosylation

Olga Gornik
contact affiliation	Department of Biochemistry and Molecular Biology, University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovačića 1, 10000 Zagreb, Croatia
contact email	ogornik@pharma.hr
lab head
Dinko Šoić
contact affiliation	University of Zagreb Faculty of Pharmacy and Biochemistry
contact email	dsoic@pharma.hr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074569

PRIDE project URI

• PRIDE
  1. PXD074569
     1. Label: PRIDE project
     2. Name: Glycoproteomic and genetic analysis of N-glycosylation of complement component C3 reveals immune pathway regulation