PXD074543 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | β-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division |
| Description | The mitotic spindle is a microtubule-based apparatus that is responsible for accurate segregation of chromosomes into two daughter cells. In this study, we show that the DNA damage kinase Chk1 is required for optimal density and efficient polymerization of spindle microtubules during unperturbed mitosis in vertebrate cells. Chk1 phosphorylates -tubulin at the newly identified site threonine-285 (T285) at mitotic centrosomes. Impaired -tubulin-T285 phosphorylation correlates with improper spindles, delayed anaphase onset, erroneous chromosome alignment and segregation, unequal daughter cell-size and reduced cell proliferation, whereas expression of a phosphomimetic -tubulin rescues spindle formation and functions in Chk1-deficient cells. The ATR-interacting protein ATRIP promotes localization of the ATR kinase and mediator protein TopBP1 to mitotic centrosomes; furthermore, interaction of ATRIP with ATR and TopBP1 is required for Chk1 activation and - tubulin-T285 phosphorylation. Also, inhibition of T285 phosphorylation synergizes with microtubule poisons to inhibit cell proliferation. These results identify a novel mechanism that promotes normal spindle formation, through Chk1-mediated - tubulin-T285 phosphorylation. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-08_01:26:41.313.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD074543 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sergio Lilla |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Spodoptera frugiperda; NCBI TaxID: NEWT:7108; |
| ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-17 04:20:14 | ID requested | |
| ⏵ 1 | 2026-06-08 01:26:42 | announced | |
Publication List
| 10.1038/s42003-026-09862-x; |
| Boutakoglou N, Petsalaki E, Balafouti S, Efthymiou D, Lilla S, Giatagana EM, Zanivan S, Zachos G, -tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division. Commun Biol, 9(1):(2026) [pubmed] |
| 10.6019/PXD074543; |
Keyword List
| submitter keyword: ATR, ATRIP, TopBP1, centrosome, chromosome segregation, -tubulin, Chk1, cell proliferation,Mitotic spindle |
Contact List
| Sara Rossana Zanivan |
|---|
| contact affiliation | CRUK -CRUK Scotland nstitute for Cancer Research - Switchback Rd, Bearsden, Glasgow G61 1BD - United Kingdom |
| contact email | s.zanivan@beatson.gla.ac.uk |
| lab head | |
| Sergio Lilla |
|---|
| contact affiliation | Proteomics |
| contact email | s.lilla@beatson.gla.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074543
- Label: PRIDE project
- Name: β-tubulin phosphorylation by Chk1 is required for normal spindle formation during cell division
