PXD074532 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Chemical Proteomics Reveals Regulation of Bile Salt Hydrolases via Oxidative Post-translational Modifications |
| Description | Bile salt hydrolase is a cysteine hydrolase that catalyzes the deconjugation of amino acids from bile acid amidates. The catalytic residue, cysteine 2, must be in a thiolate form for hydrolysis to occur. Using a chemical probe, CA-Dim (file name suffix 'D'), we identified cysteine 2 reversibly forms a sulfinic acid, whose profiling was enabled by this probe. We profiled this posttranslation modification in the murine fecal microbiome, comparing to a DMSO vehicle control (file name suffix 'C') and a thiolate-selective probe, CA-FMK (file name suffix 'F'). |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-27 |
| AnnouncementXML | Submission_2026-02-26_16:40:31.762.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kien Malarney |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-16 15:20:54 | ID requested | |
| ⏵ 1 | 2026-02-26 16:40:32 | announced | |
Publication List
| 10.1021/jacs.5c18912; |
| 10.1021/JACS.5C18912; |
| Bracken AK, Malarney KP, Chang PV, Chemical Proteomics Reveals Regulation of Bile Salt Hydrolases via Oxidative Post-translational Modifications. J Am Chem Soc, 148(5):5378-5386(2026) [pubmed] |
Keyword List
| submitter keyword: bile salt hydrolase, microbiome, chemoproteomics |
Contact List
| Pamela V. Chang |
|---|
| contact affiliation | Department of Microbiology and Immunology and Department of Chemistry and Chemical Biology, Cornell University, USA |
| contact email | pamela.chang@cornell.edu |
| lab head | |
| Kien Malarney |
|---|
| contact affiliation | Cornell University |
| contact email | kpm67@cornell.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD074532 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074532
- Label: PRIDE project
- Name: Chemical Proteomics Reveals Regulation of Bile Salt Hydrolases via Oxidative Post-translational Modifications
