PXD074528

PXD074528 is an original dataset announced via ProteomeXchange.

Title	Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16
Description	Cyclin-dependent protein kinase 16 (CDK16) regulates both physiological and pathological processes, including autophagy, spermatogenesis and cancer. Unlike other CDKs, CDK16 is regulated by an unclear mechanism involving phosphorylated cyclin Y (CCNY) in complex with 14-3-3 proteins rather than CCNY alone. The present study aims at elucidating this mechanism by structurally characterizing CDK16 in complex with CCNY and 14-3-3 using several biophysical techniques. As shown by cryo-EM analysis and hydrogen/deuterium exchange coupled to mass spectrometry, 14-3-3 binding unmasks a key moiety of the CDK binding surface of CCNY, thereby enabling CDK16 activation. CDK16 interacts with the cyclin box of CCNY, while 14-3-3 provides additional contacts, including with the activation segment of CDK16. CDK16 activation also requires interactions of CCNY with the N-terminal extension of CDK16. These findings not only clarify the role of CCNY and 14-3-3 in CDK16 activation but also highlight the potential of targeting CDK16 protein-protein interactions for cancer therapy.
HostingRepository	PRIDE
AnnounceDate	2026-03-31
AnnouncementXML	Submission_2026-03-31_09:43:34.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petr Pompach
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-16 12:54:45	ID requested
⏵ 1	2026-03-31 09:43:34	announced

10.1038/S41467-026-70778-5;

submitter keyword: structure,CDK16, cyclin Y/14-3-3

Tomas Obsil
contact affiliation	Department of Physical and Macromolecular Chemistry, Faculty of Science, Charles University, Prague, Czech Republic
contact email	tomas.obsil@natur.cuni.cz
lab head
Petr Pompach
contact affiliation	Intitute of Biotechnology
contact email	petr.pompach@ibt.cas.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD074528

PRIDE project URI

• PRIDE
  1. PXD074528
     1. Label: PRIDE project
     2. Name: Structural basis of the cyclin Y/14-3-3 protein-mediated activation of CDK16