PXD074523

PXD074523 is an original dataset announced via ProteomeXchange.

Title	Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex
Description	Limiting the synthesis and activity of ribosomes is crucial for adaptation to stresses, such as heat or nutrient starvation. In Bacillus subtilis, this can be achieved through the coordinated action of the alarmones (p)ppGpp and the transcription factor Spx. Here, we performed a genetic screen to uncover novel factors contributing to the heat shock response of B. subtilis. We identified the Y-complex, which confers specificity to the endonuclease RNase Y, as a critical player during stress conditions, such as heat or transition into stationary phase. This protein complex is required for processing diverse RNAs, notably the maturation of mRNAs encoding proteins involved in translation and metabolism. We further demonstrate that the Y- complex and RNase Y initiate the degradation of rRNAs of mature ribosomes, lowering their abundance. We propose that the Y-complex is a regulatory hub that modulates gene expression, adjusts protein synthesis and resource allocation.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_06:55:51.281.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Bacillus sp. B-23280; NCBI TaxID: NEWT:101037;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-16 11:28:30	ID requested
⏵ 1	2026-04-09 06:55:51	announced

Dataset with its publication pending

submitter keyword: None

Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens Charitéplatz 1 | D-10117 Berlin | Germany
contact email	alagesan@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074523

PRIDE project URI

• PRIDE
  1. PXD074523
     1. Label: PRIDE project
     2. Name: Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex