PXD074521

PXD074521 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation
Description	This study aims to evaluate the performance of the magnetic aptamer probe (MAP) system in the enrichment of migrasomes. First, the MAP system was constructed by functionalizing biotin-labeled migrasome-targeting aptamers (Apt_B3) onto the surface of NeutrAvidin-coated magnetic sub-micrometre particles. To gain a deeper understanding of the system’s efficacy, we collected 4 clinical plasma samples and isolated the migrasomes using both traditional density gradient centrifugation (DGC) and the MAP system respectively. Subsequently, the protein composition of the migrasomes isolated by these two methods was analyzed using quantitative proteomics. To directly evaluate the reproducibility of our MAP isolation protocol, we compared the proteomic profiles of migrasomes isolated in two independent preparation batches. Batch-1 consisted of two replicates (MAP_a and MAP_b), and Batch-2 comprised four replicates (MAP_1-4). This approach seeks to demonstrate the promising applicability of the MAP system in studies related to clinical disease diagnosis of migrasomes.
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:10:59.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yixin Xu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-16 09:11:02	ID requested
⏵ 1	2026-04-26 16:11:01	announced

10.1002/jev2.70283;

Xu Y, Jiao L, Yi Q, Chen Y, Cai B, Zhang J, Huang Z, Luo Y, Si Y, Wu Y, Ying B, Chen J, Zhou J, Magnetic-Assisted Aptamer Selection and Isolation of Migrasomes for Application in Lung Adenocarcinoma Proteomic Analysis. J Extracell Vesicles, 15(4):e70283(2026) [pubmed]

submitter keyword: Density Gradient Centrifugation, Proteomic, Aptamer,Migrasome

Yixin Xu
contact affiliation	Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
contact email	xuyixin@scu.edu.cn
lab head
Yixin Xu
contact affiliation	Department of Laboratory Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
contact email	xuyixin@scu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074521

PRIDE project URI

• PRIDE
  1. PXD074521
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of the Protein Composition of Migrasomes Enriched by Magnetic Aptamer Probe System and Density Gradient Centrifugation