PXD074514

PXD074514 is an original dataset announced via ProteomeXchange.

Title	Tumor-Associated Senescent Non-Classical Monocytes Facilitate Immune Suppression and Advanced Prostate Cancer Progression through Tumor and Host Immune Interactions
Description	Prostate cancer, a leading cause of male cancer mortality, poses major therapeutic hurdles due to its immunosuppressive tumor microenvironment (TME). Here, we link MYC amplification and PTEN deletion to prostate adenocarcinoma metastasis and identify a specific tumor-associated non-classical monocyte subset (staNC-Mo, CD11b+Ly6cLowCD14HiCD43LowCX3CR1Low) as a critical driver of TME immunosuppression. MYC overexpression combined with Pten loss induces mitochondrial stress, triggering tumor cell apoptosis and HSP60 release. HSP-60-TLR4 signaling activates staNC-Mo to secrete TNF-α, stimulating endothelial G-CSF production and driving staNC-Mo differentiation in bone marrow and spleen. CD14 expressed on staNC-Mo facilitates the phagocytosis of apoptotic tumor cells, subsequently inducing staNC-Mo senescence and promoting TGF-β1 secretion, which in turn upregulates CXCL5 secretion in tumor cells via the PI3K/AKT signaling pathway, thereby reinforcing staNC-Mo chemotaxis. Senescent staNC-Mo additionally secretes IL-18, further suppressing innate and adaptive immunity. Therapeutically, targeting Ly6c, CD14, CXCL5-CXCR2, TLR4, or IL-18 signaling pathways effectively reduces staNC-Mo chemotaxis, alleviates TME immunosuppression, and attenuates tumor progression, highlighting promising strategies to restore immune surveillance in advanced prostate cancer.
HostingRepository	PRIDE
AnnounceDate	2026-09-03
AnnouncementXML	Submission_2026-09-03_06:48:50.572.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chao Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-16 06:22:32	ID requested
⏵ 1	2026-09-03 06:48:51	announced

Dataset with its publication pending

submitter keyword: Mouse Cell lines

Runhua Liu
contact affiliation	The University of Alabama at Birmingham
contact email	chaozhang@uabmc.edu
lab head
Chao Zhang
contact affiliation	University of Alabama at Birmingham
contact email	chaozhang@uabmc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074514
     1. Label: PRIDE project
     2. Name: Tumor-Associated Senescent Non-Classical Monocytes Facilitate Immune Suppression and Advanced Prostate Cancer Progression through Tumor and Host Immune Interactions