PXD074509 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mass spectrometry analysis of in vitro PKCe phosphorylated ADSL |
| Description | Bacterially purified His-ADSL proteins on Ni-NTA agarose beads were incubated with or without active PKCε in the presence of ATP for an in vitro kinase assay. After centrifugation, the agarose beads were collected and mixed with 30 μL of SDS-PAGE sample loading buffer. The samples were then separated by SDS-PAGE and visualized by Coomassie Brilliant Blue staining. The protein bands were excised from the gel, digested with trypsin, and analyzed by LC-MS/MS using an Orbitrap Elite mass spectrometer. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-16 |
| AnnouncementXML | Submission_2026-02-16_05:58:32.898.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | yuran duan |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | phosphorylated residue |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-16 05:13:31 | ID requested | |
| ⏵ 1 | 2026-02-16 05:58:33 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: In vitro kinase assay |
| LC-MS/MS |
| phosphorylation |
Contact List
| Daqian Xu |
|---|
| contact affiliation | Institute of Translational Medicine, Zhejiang University School of Medicine, Zhejiang University |
| contact email | xudaqian@zju.edu.cn |
| lab head | |
| yuran duan |
|---|
| contact affiliation | Institute of Translational Medicine, Zhejiang University School of Medicine, Zhejiang University |
| contact email | yuranduan@zju.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074509
- Label: PRIDE project
- Name: Mass spectrometry analysis of in vitro PKCe phosphorylated ADSL
