PXD074504

PXD074504 is an original dataset announced via ProteomeXchange.

Title	Bacterial effector modifies host messenger RNA to promote virulence
Description	Bacterial pathogens harbor specialized secretion systems that inject effector proteins into the host cell to establish infection and disease. While many bacterial effectors post-translationally modify proteins to influence host responses, the extent to which effectors modify host RNA is currently unknown. Here we performed RNA-interactome capture (RIC) to isolate effectors bound to host cellular messenger RNA (mRNA) during Legionella pneumophila infection. RIC identified an uncharacterized effector, FadA (Lpw16921), which localized to the host-cell nucleus and interacted with host mRNAs at uracil (U)-rich RNA motifs. FadA exhibited NADPH-oxidase activity that mediated 8-oxo-guanine (oxo8G) modifications of mRNA substrates, resulting in oxidative damage and inhibition of translation. Infection with L. pneumophila harboring wild-type FadA, but not a catalytically inactive mutant, increased oxo8G modifications, suppressed cytokine responses, and promoted bacterial persistence in vivo. Our findings demonstrate the potential for a secreted Q2 effector to post-transcriptionally modify host mRNA as a mechanism to promote bacterial virulence.
HostingRepository	PRIDE
AnnounceDate	2026-08-15
AnnouncementXML	Submission_2026-08-14_19:30:06.910.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathleen McCaffrey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	Vanquish Flex

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-16 02:05:12	ID requested
1	2026-08-14 03:27:27	announced
⏵ 2	2026-08-14 19:30:07	announced	2026-08-15: Updated project metadata.

10.1016/j.chom.2026.07.018;

submitter keyword: Nucleoside LC MS

Elizabeth Hartland
contact affiliation	1. Centre for Innate Immunity and Infectious Diseases, Hudson Institute of Medical Research, Clayton, Victoria, Australia. 2. Department of Molecular and Translational Science, Monash University, Clayton, Victoria, Australia.
contact email	elizabeth.hartland@hudson.org.au
lab head
Kathleen McCaffrey
contact affiliation	Hudson Institute of Medical Research, Clayton, Victoria
contact email	kathleen.mccaffrey@hudson.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD074504

PRIDE project URI

• PRIDE
  1. PXD074504
     1. Label: PRIDE project
     2. Name: Bacterial effector modifies host messenger RNA to promote virulence