PXD074475

PXD074475 is an original dataset announced via ProteomeXchange.

Title	Global stabilization of the mitochondrial proteome is associated with extreme anoxia tolerance in Austrofundulus limnaeus WS40NE cells
Description	Austrofundulus limnaeus is an extremophile vertebrate native to small temporary ponds of Venezuela. Embryos of A. limnaeus must survive variable and often extreme conditions, including long periods of anoxia. Neuroepithelial cells derived from these embryos, WS40NE cells, provide a unique tool to understand how the proteome changes in response to anoxic stress. Using label-free proteomics, 19604 peptides and 3487 proteins were quantified in normoxic, 4d anoxic, and 24 h recovery WS40NE cells. Of these, 2612 proteins (74.9%) were statistically significantly differentially expressed in at least one comparison: 1988 comparing normoxia to 4d anoxia (57.0%), 923 comparing 4d anoxia to 24 h recovery (26.5%), and 1814 comparing normoxia to 24 h recovery (52.0%). Further, interaction networks of proteins with similar expression patterns suggest that relative mitochondrial capacity may increase during anoxia, including upregulation and/or preferred stabilization of proteins involved in mitochondrial metabolism and mitochondrial transcription and translation. This is in sharp contrast to trends in proteins that support cytoplasmic translation. These data support an active role for mitochondria in mediating the survival of anoxia tolerant killifish cells.
HostingRepository	PanoramaPublic
AnnounceDate	2026-08-27
AnnouncementXML	Submission_2026-08-27_18:35:39.449.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dietmar Kültz
SpeciesList	scientific name: Austrofundulus limnaeus; NCBI TaxID: 52670;
ModificationList	Acetyl; Carbamidomethyl; Oxidation
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-13 23:35:34	ID requested
⏵ 1	2026-08-27 18:35:40	announced

Hughes C, Corona MM, K, ü, ltz D, Podrabsky JE, Global stabilization of the mitochondrial proteome is associated with extreme anoxia tolerance in Austrofundulus limnaeus WS40NE cells. BMC Biol, ():(2026) [pubmed]

submitter keyword: anoxia, stress tolerance, proteomics, fish cell line

Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
lab head
Dietmar Kültz
contact affiliation	UC Davis
contact email	dkueltz@ucdavis.edu
dataset submitter

Panorama Public dataset URI