⮝ Full datasets listing
PXD074443
PXD074443 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Photoaffinity labeling of human coronavirus 229E-infected MRC5 cells |
| Description | Proteomic analysis of photoaffinity tagged methyltransferase inhibitor treated human fibroblasts infected with human coronavirus 229E. MRC5 cells were mock-infected (saline buffer treatment only) or infected with human coronavirus 229E in the presence or absence of the photoaffinity tagged methyltransferase inhibitor (10 and 40 micromolar) for 48 hours. Cells were UV treated, scraped, and lysed in 0.5% sodium dodecyl sulfate. Click chemistry was performed on samples normalized for protein concentration and samples were resolved on 10 to 20% Tris Glycine gels. Affinity probed targeted proteins were enriched using streptavidin beads prior to being submitted for proteomics. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-13 |
| AnnouncementXML | Submission_2026-02-13_16:10:19.926.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Matthew Monroe |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; scientific name: Human coronavirus 229E; NCBI TaxID: 11137; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-13 11:31:54 | ID requested | |
| ⏵ 1 | 2026-02-13 16:10:20 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: 229-E-infected MRC5, homo sapiens, LC-MS/MS, DatasetType:Proteomics |
Contact List
| Nicholas J. Reichart | |
|---|---|
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | nicholas.reichart@pnnl.gov |
| lab head | |
| Amy C. Sims | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | amy.sims@pnnl.gov |
| lab head | |
| Matthew Monroe | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | matthew.monroe@pnnl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100843/ |
