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PXD074420

PXD074420 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSoil microbiome pH perturbation proteomics
DescriptionExamined soil microbiome microcosms to determine the effect of changing pH on the production of lignocellulolytic enzymes. Soil from a Prosser, Washington field site was incubated in mesh bags on top of a soil interfacing glass bead matrix with MOPS minimal media amended with or without carboxymethyl cellulose at three different pH levels. After incubation, 2 mL of media solution was collected for proteomics, separating intracellular proteome from extracellular proteome via centrifugation. Data was searched with MS-GF+ and MASIC using PNNL's DMS Processing pipeline. Quantitative values were determined using MS1 abundance profiles created by MASIC for every MS2 spectrum, allowing for area under the curve assessments of peptide elution.
HostingRepositoryMassIVE
AnnounceDate2026-02-13
AnnouncementXMLSubmission_2026-02-13_16:09:48.689.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMatthew Monroe
SpeciesList scientific name: environmental samples ; NCBI TaxID: 48479;
ModificationListOxidation; Carbamidomethyl
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-02-13 01:06:34ID requested
12026-02-13 16:09:49announced
Publication List
no publication
Keyword List
submitter keyword: soil microbiome, DatasetType:Proteomics
Contact List
Ryan S. McClure
contact affiliationPacific Northwest National Laboratory
contact emailryan.mcclure@pnnl.gov
lab head
Nicholas J. Reichart
contact affiliationPacific Northwest National Laboratory
contact emailnicholas.reichart@pnnl.gov
lab head
Matthew Monroe
contact affiliationPacific Northwest National Laboratory
contact emailmatthew.monroe@pnnl.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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