⮝ Full datasets listing
PXD074420
PXD074420 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Soil microbiome pH perturbation proteomics |
| Description | Examined soil microbiome microcosms to determine the effect of changing pH on the production of lignocellulolytic enzymes. Soil from a Prosser, Washington field site was incubated in mesh bags on top of a soil interfacing glass bead matrix with MOPS minimal media amended with or without carboxymethyl cellulose at three different pH levels. After incubation, 2 mL of media solution was collected for proteomics, separating intracellular proteome from extracellular proteome via centrifugation. Data was searched with MS-GF+ and MASIC using PNNL's DMS Processing pipeline. Quantitative values were determined using MS1 abundance profiles created by MASIC for every MS2 spectrum, allowing for area under the curve assessments of peptide elution. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-13 |
| AnnouncementXML | Submission_2026-02-13_16:09:48.689.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Matthew Monroe |
| SpeciesList | scientific name: environmental samples |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-13 01:06:34 | ID requested | |
| ⏵ 1 | 2026-02-13 16:09:49 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: soil microbiome, DatasetType:Proteomics |
Contact List
| Ryan S. McClure | |
|---|---|
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | ryan.mcclure@pnnl.gov |
| lab head | |
| Nicholas J. Reichart | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | nicholas.reichart@pnnl.gov |
| lab head | |
| Matthew Monroe | |
| contact affiliation | Pacific Northwest National Laboratory |
| contact email | matthew.monroe@pnnl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100832/ |




