⮝ Full datasets listing
PXD074369
PXD074369 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Phosphoproteomic characterization of altered signaling pathways across hippocampus development upon loss of epilepsy-associated CDKL5 kinase |
| Description | Lesions of the X-linked cyclin-dependent kinase-like 5 (CDKL5) gene are associated with a severe developmental epileptic encephalopathy called CDKL5 deficiency disorder (CDD). CDKL5 is a serine-threonine kinase that has been implicated as a regulator of neuronal functioning, and its loss results in multiple behavioral phenotypes in animal models. One example, hippocampus-dependent learning and memory deficits, represents a clinically relevant phenotype that is reproducible across studies. To determine how losing CDKL5 alters biochemical signaling pathways in the hippocampus in vivo, we isolated whole hippocampal tissue from CDD mice for proteomic and phosphoproteomic analysis. Furthermore, CDKL5 is known to have important early developmental and post-developmental roles in the brain, so we collected tissues at both early postnatal and later adolescent timepoints to elucidate how signaling pathway alterations differ across development. Our DIA-based mass spectrometry results revealed that loss of CDKL5 alters multiple biochemical signaling pathways, with changes predominantly reflecting altered protein phosphorylation, not changes in protein abundance. Differently regulated proteins were enriched for multiple synaptic and cytoskeletal signaling pathways during and after early development. Overall, our findings highlight several biochemical signaling pathways that are altered in CDD and informs future mechanistic and therapeutic studies aiming to better understand and treat CDD. |
| HostingRepository | jPOST |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-27_17:17:39.382.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yi-Kai Liu |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-11 18:50:09 | ID requested | |
| ⏵ 1 | 2026-04-27 17:17:39 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: CDKL5 deficiency disorder, CDD, proteomics, phosphoproteomics, DIA |
Contact List
| W. Andy Tao, Zhaolan Zhou | |
|---|---|
| lab head | |
| Yi-Kai Liu | |
| contact affiliation | Purdue University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST004393/ |
