PXD074293 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mechanism of protein abundance-independent 3′UTR-regulated JMJD3 activity in human cells |
| Description | The 3′UTR in the KDM6B mRNA can control the cellular activity of the produced KDM6B/JMJD3 protein, but the mechanism is unclear. To examine whether 3′UTR-dependent JMJD3 protein complexes are responsible for the observed 3′UTR-dependent protein activity, KDM6B cDNA expression constructs with or without the KDM6B 3′UTR were transfected into HeLa cells grown in SILAC media, followed by co-IP of the HA-tagged JMJD3 protein. Cells were grown in SILAC medium for 5 passages and were validated for amino acid incorporation before the experiment. We did not detect major differences in JMJD3 protein interactors, when JMJD3 was translated from mRNA templates with or without its 3′UTR. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-01 |
| AnnouncementXML | Submission_2026-06-01_06:18:11.493.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhuoning Li |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-10 00:04:49 | ID requested | |
| ⏵ 1 | 2026-06-01 06:18:11 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
Contact List
| Christine Mayr |
|---|
| contact affiliation | Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA. |
| contact email | mayrc@mskcc.org |
| lab head | |
| Zhuoning Li |
|---|
| contact affiliation | MSKCC |
| contact email | liz2@mskcc.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD074293 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074293
- Label: PRIDE project
- Name: Mechanism of protein abundance-independent 3′UTR-regulated JMJD3 activity in human cells
