PXD074282

PXD074282 is an original dataset announced via ProteomeXchange.

Title	Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12
Description	This project aims to characterize the endothelial cell proteome labeled by tumor-derived secreted Sema3C in metastatic lymph nodes using an in vivo TurboID-based proximity labeling strategy. EO771 breast cancer cells overexpressing either a secreted p65-Sema3C–TurboID fusion protein or TurboID alone were orthotopically implanted into C57BL/6 mice. Following tumor growth and lymph node colonization, systemic biotin administration enabled proximity-dependent biotinylation of proteins in neighboring host endothelial cells. Primary high endothelial cells (HECs) were isolated from metastatic lymph nodes, and biotinylated proteins were enriched and analyzed by high-resolution data-independent acquisition (DIA) mass spectrometry. This dataset provides a resource for identifying Sema3C-associated endothelial proteins in the metastatic tumor microenvironment in vivo.
HostingRepository	iProX
AnnounceDate	2026-02-10
AnnouncementXML	Submission_2026-03-17_20:33:36.953.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qidong Xia
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-09 19:46:47	ID requested
⏵ 1	2026-03-17 20:33:37	announced

Dataset with its publication pending

submitter keyword: Proximity labeling, TurboID, Sema3C, lymph node

Shicheng Su
contact affiliation	Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University
contact email	sushch@mail.sysu.edu.cn
lab head
Qidong Xia
contact affiliation	Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University
contact email	xiaqd3@mail.sysu.edu.cn
dataset submitter

iProX dataset URI