⮝ Full datasets listing
PXD074282
PXD074282 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12 |
| Description | This project aims to characterize the endothelial cell proteome labeled by tumor-derived secreted Sema3C in metastatic lymph nodes using an in vivo TurboID-based proximity labeling strategy. EO771 breast cancer cells overexpressing either a secreted p65-Sema3C–TurboID fusion protein or TurboID alone were orthotopically implanted into C57BL/6 mice. Following tumor growth and lymph node colonization, systemic biotin administration enabled proximity-dependent biotinylation of proteins in neighboring host endothelial cells. Primary high endothelial cells (HECs) were isolated from metastatic lymph nodes, and biotinylated proteins were enriched and analyzed by high-resolution data-independent acquisition (DIA) mass spectrometry. This dataset provides a resource for identifying Sema3C-associated endothelial proteins in the metastatic tumor microenvironment in vivo. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-10 |
| AnnouncementXML | Submission_2026-05-21_00:45:05.218.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Qidong Xia |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-09 19:46:47 | ID requested | |
| 1 | 2026-03-17 20:33:37 | announced | |
| ⏵ 2 | 2026-05-21 00:45:06 | announced | 2026-05-21: Add info |
Publication List
| Xia Q, Pan J, Weng X, Li S, Li J, Song S, Li J, Zhou B, Liu X, Kuang DM, Zhang N, Jin J, Liu J, Lin Z, Yu S, Tang Q, Bian L, Zeng Y, Shi Y, Zheng Y, Liao JY, Xu S, Su S, Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12. Nat Commun, ():(2026) [pubmed] |
Keyword List
| submitter keyword: Proximity labeling, TurboID, Sema3C, lymph node |
Contact List
| Shicheng Su | |
|---|---|
| contact affiliation | Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University |
| contact email | sushch@mail.sysu.edu.cn |
| lab head | |
| Qidong Xia | |
| contact affiliation | Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University |
| contact email | xiaqd3@mail.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
