⮝ Full datasets listing
PXD074226
PXD074226 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | History recording and content mapping of inter-organelle contacts in living systems via CLAP-ID |
| Description | Organelles dynamically interact to coordinate cellular functions, yet existing methods fail to record interaction histories or resolve contact-specific molecular compositions. Here, we introduce CLAP-ID, a light-gated method for tagging and profiling contacting organelles in living cells. Using a light-activated biotin ligase targeted to one organelle, CLAP-ID biotinylates adjacent organelles during transient contacts, enabling their subsequent isolation and multi-omic analysis. Validating CLAP-ID on mitochondria-ER interactions, we identified metabolites and proteins enriched in ER-proximal mitochondria. Furthermore, bidirectional CLAP-ID mapping of mitochondria-endolysosome contacts revealed that SNX3 on endolysosomes regulates mitochondrial morphology and that calcium-binding proteins rapidly relocalize from mitochondria-lysosome contacts upon lysosomal damage. CLAP-ID quantified contact extent across over 20 organelle pairs and uncovered new peroxisome-stress granule contacts mediating protein transfer. It also resolved distinct, contact-dependent mitochondrial subtypes within mouse tumor tissues and hippocampal neurons. By enabling organelle classification based on interaction history, CLAP-ID provides a versatile tool for dissecting contact-dependent organelle regulation. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-07 |
| AnnouncementXML | Submission_2026-02-09_01:55:48.722.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hongyang Guo |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-09 01:55:12 | ID requested | |
| ⏵ 1 | 2026-02-09 01:55:49 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Proximity Labeling, Membrane contact sites, LOV-BirA |
Contact List
| Wei Qin | |
|---|---|
| contact affiliation | Tsinghua University |
| contact email | weiqin@mail.tsinghua.edu.cn |
| lab head | |
| Hongyang Guo | |
| contact affiliation | Tsinghua University |
| contact email | guohy21@mails.tsinghua.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
