⮝ Full datasets listing
PXD074225
PXD074225 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein Extraction Method Determines Proteomic Coverage and Functional Profiles in Adipose TIssue |
| Description | Adipose tissue plays a key role in metabolic and immune regulation, implying that proteomic analysis of adipose tissue is essential for understanding metabolic diseases. However, in-depth proteomics analysis of adipose tissue presents technical challenges due to its high lipid content and low protein yield. This study compared two methods for extraction proteins from adipose tissue and evaluated their physicochemical and biological properties. RELi extraction method and the LD-C2PE method showed different patterns in protein properties. RELi preferentially enhanced the detection of low abundance, high hydrophobicity and low solubility. In contrast, LD-C2PE enriched secreted and extracellular proteins associated with cellular organization and tissue architecture. Using RELi extraction method, we characterized depot-specific and diet-induced proteomic adaptations in white adipose tissue (WAT). Retroperitoneal fat was enriched for lipid metabolic and immune-related pathways, whereas mesenteric fat showed activation of RNA regulatory processes. High-fat diet induced depot-specific responses, with immunoregulatory pathways in retroperitoneal WAT and protein-RNA complex related pathways in mesenteric WAT. In summary, our findings demonstrate that protein extraction strategy is crucial for adipose tissue proteomic landscapes and that integrating extraction method, fat depot location, and dietary manner are essential for biologically meaningful interpretation of adipose tissue proteomics. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-06-02 |
| AnnouncementXML | Submission_2026-06-02_02:36:13.099.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Seong Woo Choi |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-09 00:48:29 | ID requested | |
| ⏵ 1 | 2026-06-02 02:36:14 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Adipose tissue, Proteomics, DatasetType:Proteomics |
Contact List
| Jin Young Kim | |
|---|---|
| contact affiliation | Korea Basic Science Institute |
| contact email | jinyoung@kbsi.re.kr |
| lab head | |
| Seong Woo Choi | |
| contact affiliation | Korea Advanced Institue of Science and Technology |
| contact email | wooyaachoi@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100767/ |
