PXD074215

PXD074215 is an original dataset announced via ProteomeXchange.

Title	Molecular Insights from Differential Proteomic Profiling of Premalignant Cervical Lesions and Cervical Cancer
Description	Introduction: Cervical cancer affects women worldwide, with more than 70% of cases directly related to high-risk HPV infection, such as types 16 and 18, which promote the progression of precancerous lesions to cancer. Objective: To identify differentially expressed proteins (DEPs) in biopsies from patients with HPV16+ LSIL and HPV16+ SCC compared to normal HPV-negative cervical control tissue. Methodology: Samples were processed using HPLC/MS-MS, and through independent data analysis using DIA (DIA), differential expression analysis was determined using DIANN software, bioinformatic analysis using Venn diagrams, pathway enrichment, functional interactomes, TCGA-SCC data, and validation of DEPs using western blot was performed. Results: Differential expression analyses between the comparative groups identified 1,607 proteins in LSIL, associated with processes such as cell adhesion and extracellular matrix proteins, while 1,516 were identified in SCC, associated with catalytic and transport activities. When analyzing the proteins overexpressed in LSIL (332), processes such as metabolism, immune response activation, and responses to stress and cell death are enriched. In contrast, in SCC-derived overexpressed proteins (205), proteins associated with the cell cycle, DNA damage, drug metabolism, proteasome degradation, methylation, and immune response are observed. Functional interaction analyses highlight proteins related to E5, E6, E7, but also E1. Proteomic validation was performed for two DEPs, S100A10 and TYMP, both of which were overexpressed in patients with premalignant lesions and SCC at the mRNA and protein levels. Conclusions: Proteomic analysis identified a set of differentially expressed proteins associated with the progression from premalignant lesions to squamous cell carcinoma (SCC). Although these proteins did not directly interact with HPV16 proteins, they did interact with carboplatin and paclitaxel, suggesting their potential relevance as therapeutic targets and biomarkers for tumor progression
HostingRepository	iProX
AnnounceDate	2026-02-08
AnnouncementXML	Submission_2026-08-25_00:45:04.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hmagdalena
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-08 23:05:27	ID requested
⏵ 1	2026-08-25 00:45:04	announced

Dataset with its publication pending

submitter keyword: LSIL, SCC, VPH16, proteomic, S100A10, TYMP

Berenice Illades A.
contact affiliation	Molecular Biomedicine Laboratory. Faculty of Chemical-Biological Sciences, Autonomous University of Guerrero
contact email	billades@uagro.mx
lab head
Hmagdalena
contact affiliation	Center for Genomic Sciences, National University of Mexico
contact email	magda@ccg.unam.mx
dataset submitter

iProX dataset URI