⮝ Full datasets listing
PXD074209
PXD074209 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | RUNX2 and USP16 Regulate MFRN2 Stability via Deubiquitination to Maintain Pulmonary Epithelial Barrier Integrity in sepsis-induced ALI |
| Description | Sepsis is a life-threatening condition characterized by high morbidity and mortality, with acute lung injury (ALI) being one of its most common and severe complications. However, the precise molecular mechanisms underlying ALI remain unclear. Here, we identify RUNX2 as a critical mediator of epithelial injury in sepsis-induced ALI, independent of macrophage activation. Mechanistically, lipopolysaccharide (LPS) stimulation enhances the binding of RUNX2 to the USP16 promoter, thereby transcriptionally activating USP16 expression. This activation reduces K27-linked ubiquitin chains on MFRN2 at lysine 97, leading to mitochondrial iron dyshomeostasis and promoting epithelial ferroptosis. Moreover, AHR interacts with RUNX2 to suppress its activation, thereby attenuating epithelial apoptosis. Collectively, our study uncovers a previously unrecognized mechanism by which LPS triggers epithelial cell death in ALI and suggests that targeting RUNX2 transcriptional activation may enhance epithelial resistance injury induced by sepsis. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-07 |
| AnnouncementXML | Submission_2026-02-08_20:15:50.861.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lai Wei |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-08 20:13:15 | ID requested | |
| ⏵ 1 | 2026-02-08 20:15:51 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: RUNX2, USP16 , MFRN2, ALI, deubiquitination, ferroptosis |
Contact List
| Wanli Jiang | |
|---|---|
| contact affiliation | Wuhan Univerisity |
| contact email | RM003262@whu.edu.cn |
| lab head | |
| Lai Wei | |
| contact affiliation | Wuhan Univerisity |
| contact email | laiwei1@whu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
