PXD074205

PXD074205 is an original dataset announced via ProteomeXchange.

Title	Automated Micro-Volume Secretome Profiling Enables Non-Invasive Protein Analysis from Single Embryos
Description	Cells continuously secrete proteins into the extracellular environment, forming the cellular secretome, which reflects dynamic cellular states in a biologically informative and non-invasive manner. However, conventional secretome analysis requires large sample volumes (>2 mL) , provides limited proteome depth and low throughput (<30 samples per day), thereby restricting its application. Here, we developed an automated micro-volume secretome workflow that integrates chemically modified magnetic beads with robotic liquid handling. The platform enables efficient capture of secreted proteins directly from less than 20 μL of neat conditioned medium, achieving deep secretome coverage of over 3,000 proteins and a throughput exceeding 96 samples per day with minimal perturbation to the culture system. Using this workflow, we achieved high-depth, time-resolved secretome profiling from micro-volume culture media. We also profiled single mouse embryo culture media, identifying more than 200 proteins per embryo. Notably, stage-specific embryo-secreted proteins such as Sdc4 and Ooep were detected, demonstrating the utility of this platform for both longitudinal secretome analysis and non-invasive molecular profiling at the single-embryo level.
HostingRepository	iProX
AnnounceDate	2026-02-06
AnnouncementXML	Submission_2026-04-30_00:59:08.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingsheng Xie
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-08 19:53:14	ID requested
⏵ 1	2026-04-30 00:59:09	announced

Dataset with its publication pending

submitter keyword: Secretome, Micro-Volume, Non-Invasive biopsy, Single-Embryo

Zilu Ye
contact affiliation	State Key Laboratory of Common Mechanism Research for Major Diseases, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	yzl@ism.pumc.edu.cn
lab head
Jingsheng Xie
contact affiliation	State Key Laboratory of Common Mechanism Research for Major Diseases, Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College
contact email	xjsnjmu@gmail.com
dataset submitter

iProX dataset URI