PXD074187

PXD074187 is an original dataset announced via ProteomeXchange.

Title	Proteomic signatures of Psychosocial Stress and Western Diet- driven Mitochondrial Dysfunction in Frontal Cortex and Hippocampus
Description	Chronic Stress and Western diet are major risk factors for affective disorders, however their biological interactions remain unclear. This study examined the neurochemical, mitochondrial, metabolic, behavioural and proteomic effects of a chronic stress paradigm (2h/day for 2 weeks) in male C57bl/6 mice fed either a control or Western diet for 20 weeks. This study showed that a Western diet induced metabolic dysfunction and depressive-like symptoms (reduce sucrose preference), but had minimal impact of frontal cortex or hippocampal mitochondrial or neurochemical measures. In contrast, chronic stress produced anxiety-like behaviour (via open field test measures), frontal cortex reductions in BDNF and GABA, elevated Glutamate and marked mitochondrial dysfunction. When combined, mice showed depressive-like behaviours despite similar neurochemical changes to that observed with stress alone. Proteomic analyses showed stress-related disruption of mitochondrial, synaptogenesis and NRF2 pathways, as well as adaptive responses to stress or Western diet that were reversed to maladaptive under co-morbid conditions. Finding implicate failed mitochondrial and synaptic adaptations in frontal cortex as key mechanisms linking stress-diet interactions to affective pathology.
HostingRepository	PRIDE
AnnounceDate	2026-05-25
AnnouncementXML	Submission_2026-05-24_16:14:24.400.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chul-Kyu Kim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	mono N-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-07 21:42:40	ID requested
⏵ 1	2026-05-24 16:14:25	announced

Helman T, Nicholas M, Lyon B, Naghipour S, Kim CK, Ybanez T, Robertson K, Griffith TA, Peart JN, Stapelberg NJC, Headrick JP, Du Toit EF, Neurobiology of co-morbid stress and a western diet in mice: mitochondrial, proteomic and behavioral outcomes. Metab Brain Dis, 41(1):(2026) [pubmed]

10.1007/s11011-026-01855-3;

submitter keyword: Depression, Mitochondria, Stress, Mouse, Respiration, Synaptogenesis,Anxiety, Western diet, Co-morbidity

Eugene Du Toit
contact affiliation	School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, Australia
contact email	j.dutoit@griffith.edu.au
lab head
Chul-Kyu Kim
contact affiliation	Centre for Healthy Brain Ageing (CHeBA), University of New South Wales, UNSW Sydney, NSW, Australia
contact email	chulkyu.kim@unsw.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD074187

PRIDE project URI

• PRIDE
  1. PXD074187
     1. Label: PRIDE project
     2. Name: Proteomic signatures of Psychosocial Stress and Western Diet- driven Mitochondrial Dysfunction in Frontal Cortex and Hippocampus