PXD074184

PXD074184 is an original dataset announced via ProteomeXchange.

Title	The MNV-1 ProPol precursor Cleaves a Novel Site in the NS1-2 Protein
Description	Human noroviruses (HuNV) are members of the Caliciviridae family and are a significant cause of gastroenteritis worldwide. Cell culture models are challenging for studying the intracellular replication of HuNV, with murine norovirus (MNV) routinely used to study norovirus replication. Norovirus genomes contain a non-structural (NS) polyprotein that is processed by the viral protease generating precursor and mature proteins during replication. We identified a putative viral protease cleavage site at E66/G67 in the MNV-1 NS1-2 protein by sequence analysis that was cleaved by the precursor ProPol but not Pro in vitro and during MNV-1 replication. We used mass spectrometry to confirm the cleavage site and the protease activity being specific to ProPol but not Pro. We used the TAILS method (Kleifeld et al. Nat Protoc. 2011;6(10):1578-611) with iTRAQ labelling to identify new N-termini after protease cleavage and data-dependent acquisition mass spectrometry as well as parallel reaction monitoring of tryptically digested gel bands of cleavage products to confirm the new N-terminus at E66/G67 in the MNV-1 NS1-2 protein.
HostingRepository	PRIDE
AnnounceDate	2026-06-08
AnnouncementXML	Submission_2026-06-07_16:08:48.326.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Torsten Kleffmann
SpeciesList	scientific name: Murine norovirus 1; NCBI TaxID: NEWT:223997;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Orbitrap Exploris 240; TripleTOF 5600; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-07 18:32:20	ID requested
⏵ 1	2026-06-07 16:08:49	announced

Ward VK, McKenzie-Goldsmith GM, Edwards MJ, Young VL, Kleffmann T, Stubbing LA, Siow A, Brimble MA, The MNV-1 protease-polymerase precursor cleaves a novel site in the NS1-2 protein. J Gen Virol, 107(4):(2026) [pubmed]

10.1099/jgv.0.002263;

submitter keyword: Norovirus, TAILS, non-structural polyprotein, protease cleavage

Vernon Ward
contact affiliation	Department of Microbiology and Immunology, University of Otago, New Zealand
contact email	vernon.ward@otago.ac.nz
lab head
Torsten Kleffmann
contact affiliation	Department of Biochemistry, University of Otago
contact email	torsten.kleffmann@otago.ac.nz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074184
     1. Label: PRIDE project
     2. Name: The MNV-1 ProPol precursor Cleaves a Novel Site in the NS1-2 Protein