PXD074107

PXD074107 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of potential emphysema biomarkers in blood plasma (EmphyseMARK-1)
Description	We conducted a small cross-sectional proteomic study aiming to identify potential biomarkers of emphysema, as well as to identify COPD- and smoking-related biomarkers. This study was approved by the University Hospital Dubrava's Institutional Ethics Committee (approval no. 2022/2908‐05), as well as by the School of Medicine, University of Zagreb's Institutional Ethics Committee (approval no. 251-59-10106-24-111/119). This study included a total of 40 participants, divided into four equal age and gender-matched groups (N=10 participants per group): 1.) patients with COPD and radiologically verified emphysema on chest CT (CE group), confirmed by a radiologist, 2.) patients with COPD without radiological signs of emphysema on chest CT (CN group), confirmed by a radiologist, 3) healthy smokers (active smokers (>20 pack-years) without respiratory symptoms and normal spirometry) (HS group), 4) healthy never-smokers (without respiratory symptoms and with normal spirometry) (HN group). A graphic study outline is shown in Figure 1. Participants were included in the study in a non-probabilistic manner (in order to be age, gender, comorbidity and body mass index matched – so group homogeneity in these regards could be achieved). Patients with COPD (CE and CN groups) were GOLD 2B patients as defined by GOLD 2023, active smokers (>20 pack-years), on dual inhalation therapy (combination of long-acting β2 agonist (LABA) and long-acting muscarinic antagonist (LAMA)). Exclusion criteria were: reversible airflow limitation, positive bronchodilator test (either by the GINA criteria or by the ERS/ATS technical standard 2022; acute exacerbation of COPD in the last 6 months, concomitant malignancy, autoimmune disease or concomitant asthma, as well as alpha-1-antitrypsin deficiency. Patients that consumed tobacco products other than classical cigarettes (including e-cigarettes, heat not-burn tobacco products, vape, cigars and cigarillos) were also excluded from the study. None of the participants were taking glucocorticoids or other immunosuppressive medications. All subjects in the study signed an informed consent form. Comorbidities were assessed using the Charlson's comorbidity index and also a modified Charlson's comorbidity index in which “chronic pulmonary disease“ was excluded. The participants underwent spirometry testing and all COPD patients had a chest CT no older than 3 months prior to inclusion. Spirometry with a subsequent bronchodilator test (with 400mcg of salbutamol) was performed according to the international standards, using the Global Lung Initiative (GLI) reference values. Expiratory airflow limitation was determined using the proposed GOLD criteria with a fixed FEV1/FVC ratio of 0.7.
HostingRepository	PRIDE
AnnounceDate	2026-04-06
AnnouncementXML	Submission_2026-04-05_16:10:59.911.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Václav Pustka
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-05 14:15:34	ID requested
⏵ 1	2026-04-05 16:11:00	announced

10.3390/diagnostics16060931;

Salai G, Novak R, Hrka, č S, Pustka V, Pot, ě, š, il D, Zdr, á, hal Z, Ljubicic D, Grgurevi, ć L, Proteomics-Based Study of Potential Emphysema Biomarkers Reveals Systemic Redox System and Extracellular Matrix Component Dysregulation. Diagnostics (Basel), 16(6):(2026) [pubmed]

submitter keyword: None

Zbynek Zdrahal
contact affiliation	Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic
contact email	zdrahal@sci.muni.cz
lab head
Václav Pustka
contact affiliation	Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic
contact email	vaclav.pustka@ceitec.muni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD074107

PRIDE project URI

• PRIDE
  1. PXD074107
     1. Label: PRIDE project
     2. Name: Proteomic study of potential emphysema biomarkers in blood plasma (EmphyseMARK-1)