PXD074106

PXD074106 is an original dataset announced via ProteomeXchange.

Title	Understanding the impact of multigene engineering on the proteome: omega-3 Camelina as a case study
Description	Camelina sativa (hereafter Camelina) has emerged as a promising platform for metabolic engineering due to its short lifecycle, high seed oil content, and amenability to transformation. Previous work has demonstrated the successful introduction of non‑native pathways for the synthesis of very long chain polyunsaturated fatty acids (VLC‑PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). These achievements required the coordinated expression of up to seven heterologous genes encoding desaturases and elongases from microalgae and other marine organisms. Although the accumulation of EPA and DHA in Camelina seeds has been well characterised, the impact of introducing such a complex pathway on endogenous seed metabolism remains largely unexplored. Here, we apply these two quantitative proteomics strategies to characterise the seed proteomes of elite EPA‑ and DHA‑producing Camelina lines. Our goals were to: 1. assess global proteomic changes associated with multigene engineering. 2. identify proteomic signatures associated with the differing oil‑yield penalties observed in EPA and DHA lines. 3. quantify the (relative) abundance of transgene‑encoded enzymes relative to endogenous lipid biosynthetic proteins.
HostingRepository	PRIDE
AnnounceDate	2026-08-14
AnnouncementXML	Submission_2026-08-14_08:25:03.489.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hongtao Zhang
SpeciesList	scientific name: Camelina sativa (False flax) (Myagrum sativum); NCBI TaxID: NEWT:90675;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-05 13:53:31	ID requested
⏵ 1	2026-08-14 08:25:04	announced

Zhang H, Napier JA, Theodoulou FL, Assessing the Impact of Multigene Engineering on the Proteome: Omega-3 Camelina as a Case Study. Plant Biotechnol J, ():(2026) [pubmed]

10.1111/pbi.70712;

submitter keyword: Camelina DHA1 EPA8 DIA

Hongtao Zhang
contact affiliation	Engineering Biology for Agriculture, Rothamsted Research,Harpenden, AL5 2JQ, UK
contact email	Hongtao.Zhang@rothamsted.ac.uk
lab head
Hongtao Zhang
contact affiliation	Rothamsted Research, Harpenden, AL5 2JQ, UK
contact email	hongtao.zhang@rothamsted.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD074106
     1. Label: PRIDE project
     2. Name: Understanding the impact of multigene engineering on the proteome: omega-3 Camelina as a case study