⮝ Full datasets listing
PXD074069
PXD074069 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Ubiquitination site mapping of NICD-interacting proteins in mouse by IP-MS |
| Description | Primary murine γδ T cells were isolated and polarized toward a pro-inflammatory γδT17 phenotype by stimulation with recombinant cytokines IL-1β (20 ng/mL), IL-23 (10 ng/mL), and IL-7 (10 ng/mL) for 72 hours. The Notch intracellular domain (NICD)-associated protein complex was then immunoprecipitated from these activated γδ T cells using an anti-NICD antibody. The immunoprecipitated material was subjected to ubiquitin remnant motif (K-ε-GG) enrichment and analyzed by high-resolution LC-MS/MS on a Q Exactive HF-X mass spectrometer to identify ubiquitination sites on NICD-interacting proteins in the context of γδT17 cell activation. |
| HostingRepository | iProX |
| AnnounceDate | 2026-02-04 |
| AnnouncementXML | Submission_2026-02-04_18:18:26.564.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yue He |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | ubiquitination signature dipeptidyl lysine |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-04 18:18:10 | ID requested | |
| ⏵ 1 | 2026-02-04 18:18:27 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: NICD Ubiquitination γδ T cell |
Contact List
| Yue Dai | |
|---|---|
| contact affiliation | China Pharmaceutical University |
| contact email | yuedaicpu@cpu.edu.cn |
| lab head | |
| Yue He | |
| contact affiliation | China Pharmaceutical University |
| contact email | 826862386@qq.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
