⮝ Full datasets listing
PXD074063
PXD074063 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | P53 and cell cycle control by an intracellular form of complement Factor H |
| Description | Overexpression of complement genes in the tumor microenvironment, including Factor H (FH), has emerged as a strong predictor of poor patient prognosis. While its canonical role as a negative regulator of the complement cascade is well-known, this alone cannot fully account for this prognostic impact. This study reveals that FH operates in a cascade-independent fashion within fibroblasts and clear cell renal cell carcinoma (ccRCC) tumor cells, where it is overexpressed under pro-tumoral inflammatory conditions. FH prognostic impact stems from its intracellular functions in tumor-promoting fibroblasts and malignant cells. FH, which has a nuclear localization signal, was found in the nucleus, cytosol and organelle fractions of both fibroblasts and ccRCC cells. Co-immunoprecipitation and mass spectrometry identified FH interactions with the cell cycle-transcription factor E2F3. FH accelerates cell cycle progression by preventing p53 activation, potentially through blocking E2F3-p53 interactions at the G1/S transition. In addition, in tumor cells, FH interacts with CapZ to regulate actin cytoskeleton. Finally, transcriptional trajectory analyses of ccRCC malignant cells revealed a CFH enriched cell state linked to proliferation and poor patient prognosis, consistent with increased Ki67 staining in FH-positive cancer cells from patient tumors. Together, these results position FH as a novel tumor-promoting effector of cell cycle through modulation of p53. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-02-04 |
| AnnouncementXML | Submission_2026-02-04_13:24:41.218.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mohamed Ali Jarboui |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl; Met-loss |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-02-04 13:02:56 | ID requested | |
| ⏵ 1 | 2026-02-04 13:24:41 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: DIA, Mass Spectrometry, Factor H, IPMS, DatasetType:Proteomics |
Contact List
| Mohamed Ali Jarboui | |
|---|---|
| contact affiliation | University klinikum Tuebingen |
| contact email | mohamed-ali.jarboui@uni-tuebingen.de |
| lab head | |
| Mohamed Ali Jarboui | |
| contact affiliation | University klinikum Tuebingen |
| contact email | mohamed-ali.jarboui@uni-tuebingen.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000100720/ |
