PXD074057 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replication |
| Description | Human cytomegalovirus (HCMV) is a leading cause of congenital infection and morbidity in immunosuppressed populations. Like all viruses, HCMV is an obligate intracellular parasite that extensively remodels host cellular metabolism to support its replication, yet the precise underlying mechanisms and metabolic vulnerabilities remain poorly understood. Using a novel metabolism-focused screening platform, we identified EGLN prolyl hydroxylase activity as critical for HCMV infection. Our studies revealed that HCMV infection depends on EGLN1, which accumulated in mitochondria during infection. Inhibition of EGLN1 expression blocked HCMV-mediated mitochondrial activation, which in turn prevented the production of the dNTP precursors necessary for dNTP pool expansion and viral DNA replication. Further, pharmacological EGLN inhibition attenuated viral infection in a humanized mouse model. Collectively, these data establish EGLN1 as a critical determinant of mitochondrial metabolic remodeling and virally-induced dNTP generation during HCMV infection, highlighting EGLN1 as a promising novel antiviral therapeutic target. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-01 |
| AnnouncementXML | Submission_2026-05-31_16:08:24.235.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Lucas Simpson |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-02-04 08:42:03 | ID requested | |
| ⏵ 1 | 2026-05-31 16:08:25 | announced | |
Publication List
| Simpson LA, Dunn DM, Fales W, Moore ZJ, Ciesla JH, Waild NC, Raymonda MH, Harris IS, Munger J, HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replication. Cell Rep, 45(5):117329(2026) [pubmed] |
| 10.1016/j.celrep.2026.117329; |
Keyword List
| submitter keyword: MRC5,HCMV, EGLN1, EGLN, Adaptaquin, LC-MS/MS |
Contact List
| Joshua Munger |
|---|
| contact affiliation | Biochemistry and Molecular Biology, Munger Lab, University of Rochester, USA |
| contact email | joshua_munger@urmc.rochester.edu |
| lab head | |
| Lucas Simpson |
|---|
| contact affiliation | University of Rochester |
| contact email | lucas_simpson@urmc.rochester.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD074057
- Label: PRIDE project
- Name: HCMV infection depends on EGLN1-mediated mitochondrial activation to increase dNTP pools for viral DNA replication
