PXD074046

PXD074046 is an original dataset announced via ProteomeXchange.

Title	Spirochaeta africana RNAP holoenzyme
Description	Crosslinking mass spectrometry was applied to investigate the localization of σR1.1 domain in the structure of Spirochaeta africana RNA polymerase holoenzyme. RNA polymerase holoenzymes were assembled using polymerase core subunits and σ factor heterologously expressed in Escherichia coli. Two complexes were assembled in the absence of S. africana CarD activator and two in the presence of CarD activator. The resulting four complexes were crosslinked using disuccinimidyl sulfoxide (DSSO). Crosslinked protein complexes were analyzed by LC-MS/MS, and crosslinked residue pairs were identified in the form of crosslinked peptides.
HostingRepository	jPOST
AnnounceDate	2026-05-01
AnnouncementXML	Submission_2026-04-30_08:00:16.749.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Georgi Belogurov
SpeciesList	scientific name: Spirochaeta africana; NCBI TaxID: 46355;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification; L-methionine sulfoxide
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-02-04 02:09:07	ID requested
⏵ 1	2026-04-30 08:00:17	announced

Dataset with its publication pending

submitter keyword: RNA polymerase, sigma factor

Georgiy A. Belogurov
lab head
Georgi Belogurov
contact affiliation	University of Turku
dataset submitter

jPOST dataset URI

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